{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:59:36Z","timestamp":1785387576222,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:00:00Z","timestamp":1785283200000},"content-version":"vor","delay-in-days":209,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100006190","name":"Research and Development","doi-asserted-by":"publisher","award":["66\/2025\/H\u0110-DHYD"],"award-info":[{"award-number":["66\/2025\/H\u0110-DHYD"]}],"id":[{"id":"10.13039\/100006190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      has emerged as an important opportunistic pathogen due to its increasing resistance to carbapenems, which severely limits therapeutic options. This study is aimed at characterizing the phenotypic resistance profiles and distribution of carbapenemase\u2010encoding genes among clinical\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      isolates collected from a tertiary\u2010care hospital in southern Vietnam.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A retrospective study was conducted on 156\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      isolates collected at the University Medical Center Ho Chi Minh City between January and December 2024. Antimicrobial susceptibility testing was performed using the BD Phoenix M50 system. Carbapenemase\u2010encoding genes, including\n                      <jats:italic>bla<\/jats:italic>\n                      OXA\u201023,\n                      <jats:italic>bla<\/jats:italic>\n                      OXA\u201058,\n                      <jats:italic>bla<\/jats:italic>\n                      NDM\u20101, and\n                      <jats:italic>bla<\/jats:italic>\n                      KPC, were detected using multiplex real\u2010time PCR.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among the 156 isolates, 70.51% were identified as carbapenem\u2010resistant\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      (CRAB). Most CRAB isolates originated from respiratory specimens (83.64%) and intensive care units (60%). CRAB isolates exhibited extensive multidrug resistance, with resistance rates ranging from 76% to &gt; 96% for most cephalosporins, fluoroquinolones, and aminoglycosides. In contrast, minocycline retained the highest in vitro activity, with a resistance rate of only 10%. The most prevalent carbapenemase gene was\n                      <jats:italic>bla<\/jats:italic>\n                      OXA\u201023 (73.6%), followed by\n                      <jats:italic>bla<\/jats:italic>\n                      NDM\u20101 (52.7%) and\n                      <jats:italic>bla<\/jats:italic>\n                      OXA\u201058 (20.0%). Overall, 95.45% of CRAB isolates were phenotypically identified as carbapenemase\u2010producing\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      (CP\u2010AB). The co\u2010occurrence of\n                      <jats:italic>bla<\/jats:italic>\n                      OXA\u201023 and\n                      <jats:italic>bla<\/jats:italic>\n                      NDM\u20101 was the most common gene combination, detected in 23.64% of CRAB isolates.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      This study demonstrates a high burden of CRAB in a Vietnamese tertiary hospital, largely driven by the co\u2010existence of\n                      <jats:italic>bla<\/jats:italic>\n                      OXA\u201023 and\n                      <jats:italic>bla<\/jats:italic>\n                      NDM\u20101. The identification of carbapenem\u2010resistant isolates lacking phenotypic carbapenemase activity suggests the involvement of alternative, nonenzymatic resistance mechanisms. Continuous molecular surveillance and strengthened antimicrobial stewardship are urgently needed to limit CRAB dissemination in Vietnam.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijm\/7420170","type":"journal-article","created":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T04:09:17Z","timestamp":1785384557000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phenotypic and Genotypic Characterization of Carbapenem\u2010Resistant\n                    <i>Acinetobacter baumannii<\/i>\n                    Clinical Isolates: Co\u2010Existence of\n                    <i>bla<\/i>\n                    OXA\u201023 and\n                    <i>bla<\/i>\n                    NDM\u20101 in a Vietnamese Tertiary Hospital"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9868-3430","authenticated-orcid":false,"given":"Tuan","family":"Huynh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9326-6942","authenticated-orcid":false,"given":"Quynh","family":"Chau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-5365-5678","authenticated-orcid":false,"given":"Loan","family":"Luong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3447-6090","authenticated-orcid":false,"given":"Kieu","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diep","family":"Bui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-7972-717X","authenticated-orcid":false,"given":"Tinh","family":"Pham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1672-3292","authenticated-orcid":false,"given":"Van","family":"Pham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6542-6147","authenticated-orcid":false,"given":"My Dung","family":"Jusselme","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.3810\/pgm.2008.09.suppl52.280"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjph-2024-002411"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.29261\/pakvetj\/2025.254"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/2475067"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/375821"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.49.8.3198-3202.2005"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.2268"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2005.08.014"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-022-00536-0"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jgar.2021.12.017"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.meegid.2019.103986"},{"key":"e_1_2_14_12_2","volume-title":"WHO Bacterial Priority Pathogens List, 2024","author":"World Health Organization","year":"2024"},{"key":"e_1_2_14_13_2","volume-title":"Antibiotic Resistance Threats in the United States, 2019","author":"Health UDo, Services H. 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