{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:54:23Z","timestamp":1791269663498,"version":"4.1.0"},"reference-count":99,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:00:00Z","timestamp":1791158400000},"content-version":"vor","delay-in-days":277,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100015690","name":"Universitas Padjadjaran","doi-asserted-by":"publisher","award":["2200\/UN6.M\/PT.01.03\/2025"],"award-info":[{"award-number":["2200\/UN6.M\/PT.01.03\/2025"]}],"id":[{"id":"10.13039\/501100015690","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Microbial conditions within sea turtle nesting environments may influence egg health and hatching outcomes, yet information on culturable bacteria associated with sea turtle eggs and nest sediments in Indonesia remains limited. This study characterized culturable bacteria from eggshell surfaces of green turtles (\n                    <jats:italic>Chelonia mydas<\/jats:italic>\n                    ), olive ridley turtles (\n                    <jats:italic>Lepidochelys olivacea<\/jats:italic>\n                    ), unhatched eggs, and natural and seminatural hatchery sediments at Batu Hiu Beach, Pangandaran, Indonesia. Three eggs were sampled from each egg category, with eggs within each category originating from the same nest. Bacterial recovery was evaluated using selective and non\u2010selective media, followed by colony characterization, Gram staining, and 16S rRNA sequencing of 21 representative isolates. Vibrio\u2010related isolates were screened for toxR, tdh, and trh genes using PCR. Green turtle eggshell swabs showed the highest mean culturable bacterial count (2,358\u2009\n                    <jats:italic>C<\/jats:italic>\n                    <jats:italic>F<\/jats:italic>\n                    <jats:italic>U<\/jats:italic>\n                    \/\n                    <jats:italic>m<\/jats:italic>\n                    <jats:italic>L<\/jats:italic>\n                    ), followed by olive ridley eggshell swabs (1,640\u2009\n                    <jats:italic>C<\/jats:italic>\n                    <jats:italic>F<\/jats:italic>\n                    <jats:italic>U<\/jats:italic>\n                    \/\n                    <jats:italic>m<\/jats:italic>\n                    <jats:italic>L<\/jats:italic>\n                    ), whereasile hatchery sediment showed the lowest mean count (109 CFU\/mL). Bacterial recovery differed among culture media (Friedman\n                    <jats:italic>\u03c7<\/jats:italic>\n                    2 = 10.47,\n                    <jats:italic>p<\/jats:italic>\n                    = 0.0332; Kendall\u2032\u2019s\n                    <jats:italic>W<\/jats:italic>\n                    = 0.436). Molecular analysis showed affiliations with Enterobacteriaceae, Vibrio, and other environmental bacterial groups. All five Vibrio\u2010related isolates were positive for toxR, whereas tdh and trh were not detected.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/7422673","type":"journal-article","created":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T06:38:01Z","timestamp":1791268681000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Identification of Culturable Bacteria and\n                    <i>Vibrio<\/i>\n                    Virulence\u2010Related Genes Associated With Sea Turtle Eggshells and Nest Sediments in Pangandaran, Indonesia"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5728-0029","authenticated-orcid":false,"given":"Titin","family":"Herawati","sequence":"first","affiliation":[{"name":"Master of Marine Conservation Universitas Padjadjaran  Sumedang West Java Indonesia  unpad.ac.id"},{"name":"Department of Marine Science Universitas Padjadjaran  Sumedang West Java Indonesia  unpad.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-3089-7023","authenticated-orcid":false,"given":"Indriyani","family":"Rahayu","sequence":"additional","affiliation":[{"name":"Doctoral Program in Sustainable Fisheries and Marine Sciences Faculty of Fisheries and Marine Sciences Universitas Padjadjaran  Sumedang Indonesia  unpad.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4269-7689","authenticated-orcid":false,"given":"Aisyah","family":"Aisyah","sequence":"additional","affiliation":[{"name":"Department of Fisheries Universitas Padjadjaran  Sumedang West Java Indonesia  unpad.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7844-9949","authenticated-orcid":false,"given":"Mochamad Untung Kurnia","family":"Agung","sequence":"additional","affiliation":[{"name":"Department of Marine Science Universitas Padjadjaran  Sumedang West Java Indonesia  unpad.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5765-3027","authenticated-orcid":false,"given":"Mochamad Rudyansyah","family":"Ismail","sequence":"additional","affiliation":[{"name":"Department of Marine Science Universitas Padjadjaran  Sumedang West Java Indonesia  unpad.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4758-0538","authenticated-orcid":false,"given":"Donny Juliandri","family":"Prihadi","sequence":"additional","affiliation":[{"name":"Department of Marine Science Universitas Padjadjaran  Sumedang West Java Indonesia  unpad.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8639-6688","authenticated-orcid":false,"given":"Eri","family":"Bachtiar","sequence":"additional","affiliation":[{"name":"Department of Marine Science Universitas Padjadjaran  Sumedang West Java Indonesia  unpad.ac.id"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9732-1676","authenticated-orcid":false,"given":"Adiana","family":"Ghazali","sequence":"additional","affiliation":[{"name":"Marine Science Faculty of Science &amp; 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