{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T21:26:10Z","timestamp":1788989170394,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":63,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Abstract<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Cutibacterium acnes<\/jats:italic>\n                      is an anaerobic bacterium isolated from prosthetic joint infections (PJI), an infection which does not induce clinically relevant symptoms for patients without fever, serum inflammatory markers and has a very indolent course.\n                      <jats:italic>C. acnes<\/jats:italic>\n                      species participates in the balance of skin microbiota but is also responsible for infections; this species is regarded as an opportunistic pathogen or pathobiont. The aim of this study was to determine the existence of a correlation between clinical infectious characteristics of patients and\n                      <jats:italic>C. acnes<\/jats:italic>\n                      clinical strains behaviors. They were evaluated through the determination of bacterial internalization, persistence rate into osteoblast\u2010like cells, and biofilm formation capacity before interaction and for internalized bacteria. This phenomenon could play a role in infections without having yet been observed\n                      <jats:italic>in vivo<\/jats:italic>\n                      . A total of 28 clinical strains were isolated and analyzed from patients with\n                      <jats:italic>C. acnes<\/jats:italic>\n                      PJI. Similar infectious clinical characteristics were observed among the PJI patients, whereas the associated clinical strains have various and heterogeneous behaviors in the\n                      <jats:italic>in vitro<\/jats:italic>\n                      assay of this study. Most of the tested\n                      <jats:italic>C. acnes<\/jats:italic>\n                      strains (75%) were internalized into osteoblast\u2010like cells with a higher rate of\n                      <jats:italic>C. acnes<\/jats:italic>\n                      strains with phylotype IA1 than other phylotypes (IB and II). High internalization rates of\n                      <jats:italic>C. acnes<\/jats:italic>\n                      in osteoblast\u2010like cells seemed to be associated with strains isolated from patients with no local inflammatory symptoms, especially articular stiffness profile. All the strains were able to form biofilm, and internalization into osteoblast\u2010like cells modified the capacity of clinical strains to form biofilm significantly for seven clinical strains (25%), associated with the presence of a high level of polymorphonuclear leukocytes\u2010patient blood with PJI from whom these strains were isolated. In our cohort, the persistence rate of\n                      <jats:italic>C. acnes<\/jats:italic>\n                      strains in osteoblast cells is less important for strains isolated from patients with tobacco use. This study raises the hypothesis that the interaction between bone environment, host, and strain modulates\n                      <jats:italic>C. acnes<\/jats:italic>\n                      ability to stimulate inflammatory symptoms in patients with\n                      <jats:italic>C. acnes<\/jats:italic>\n                      PJI.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Trial Registration<\/jats:title>\n                    <jats:p>ClinicalTrials.gov identifier: NCT03950063<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijm\/8475639","type":"journal-article","created":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:56:37Z","timestamp":1773028597000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Unraveling the Enigmatic Behavior of\n                    <i>Cutibacterium acnes<\/i>\n                    : Exploring Clinical Correlations and Behaviors of Clinical Strains in Prosthetic Joint 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