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The disruption of skin integrity elevates the risk of infection, which can impede healing and potentially lead to sepsis. Furthermore, antibiotic resistance, primarily driven by biofilm formation, poses a major challenge to effective treatment. This study is aimed at evaluating the potential of positively charged carbon dots (CDs) in inhibiting biofilm formation, with possible applications in wound care. Specifically, carbon nanodots (CDs\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    ) were tested in vitro against both planktonic cells and biofilms formed by a range of pathogens, including the Gram\u2010positive bacterium\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , the Gram\u2010negative bacterium\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    , the yeast\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    , and the mold\n                    <jats:italic>Aspergillus brasiliensis<\/jats:italic>\n                    . Additionally, the activity of CDs\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    was assessed against polymicrobial biofilms composed of\n                    <jats:italic>S. aureus<\/jats:italic>\n                    and\n                    <jats:italic>C. albicans<\/jats:italic>\n                    . The in vivo efficacy of CDs\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    was evaluated using the\n                    <jats:italic>Galleria mellonella<\/jats:italic>\n                    burn wound infection model for both monomicrobial and polymicrobial infections. The ability of CDs\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    to penetrate fungal cells was demonstrated by fluorescence microscopy analysis. Biomass quantification showed that CDs\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    reduced biofilm formation by over 50% for\n                    <jats:italic>C. albicans<\/jats:italic>\n                    ,\n                    <jats:italic>E. coli<\/jats:italic>\n                    , and\n                    <jats:italic>A. brasiliensis<\/jats:italic>\n                    , as well as for\n                    <jats:italic>C. albicans<\/jats:italic>\n                    \u2013\n                    <jats:italic>S. aureus<\/jats:italic>\n                    cocultures, at concentrations below 62.5\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    g\/mL. The in vivo studies further confirmed the antimicrobial activity of CDs\u2010NH\n                    <jats:sub>2<\/jats:sub>\n                    against all tested strains in burn wound infections. Strategies that target biofilm\u2010forming microorganisms at wound sites may enhance infection control and promote wound healing.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/8824725","type":"journal-article","created":{"date-parts":[[2025,10,14]],"date-time":"2025-10-14T12:45:39Z","timestamp":1760445939000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Amino\u2010Functionalized Carbon Nanodots Inhibit Biofilms and Infections in a Burn Wound Model, Both Caused by\n                    <i>Staphylococcus aureus<\/i>\n                    ,\n                    <i>Escherichia coli<\/i>\n                    ,\n                    <i>Candida albicans<\/i>\n                    , and\n                    <i>Aspergillus 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