{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:59:36Z","timestamp":1781161176455,"version":"3.54.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,10]],"date-time":"2026-06-10T00:00:00Z","timestamp":1781049600000},"content-version":"vor","delay-in-days":160,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    and Bovine Herpesvirus Type 1 (BoHV\u20101) are two significant pathogens of dairy cattle that cause debilitating diseases in the respiratory, reproductive, and mammary gland systems. Conventional antimicrobials have become less effective due to the emergence of antimicrobial\u2010resistant strains, and other molecules are needed for therapeutic purposes. This study was designed to identify\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    and BoHV\u20101 infection in dairy cows and to assess the antimicrobial activity of flavonoids derived from plants as alternative drugs. A total of 50 samples were collected, including 40 milk samples from cows with clinical mastitis and 10 nasal swabs from cattle with respiratory symptoms between December 12, 2023, and April 15, 2024. Mastitis\u2010associated\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    was recovered from 15\/40 milk samples (37.5%), and BoHV\u20101 was found in nasal swabs of 50% of the animals. The molecular docking studies found that the binding affinity of quercetin with\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    OmpA (\u22128.2\u2009kcal\/mol) was higher than the binding affinity of cefotaxime with the same OmpA (\u22126.9\u2009kcal\/mol). Kaempferol proved to have dual inhibitory activity against both pathogens (\u22127.5\u2009kcal\/mol). All isolates contained a conserved G\u2192T transversion at Position 21, which is based on multiple sequence alignment with the BoHV\u20101 gB reference sequence MG407791.1. The results indicate that flavonoids, specifically quercetin and kaempferol, showed good binding properties with the targeted bacteria and viruses and warrant further research to investigate their therapeutic potential.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/8888183","type":"journal-article","created":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T06:35:45Z","timestamp":1781159745000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Characterization and Molecular Docking of Flavonoid Compounds Against\n                    <i>Klebsiella pneumoniae<\/i>\n                    and Bovine Herpesvirus Type 1 (BoHV\u20101) Isolated From Clinical Dairy Cattle Cases"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2411-7871","authenticated-orcid":false,"given":"Karrar Jasim","family":"Hamzah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noor R.","family":"Abady","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4413-3062","authenticated-orcid":false,"given":"Mohammed Zuhair","family":"Naji","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2196-4039","authenticated-orcid":false,"given":"Shamsaldeen Ibrahim","family":"Saeed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3092-1201","authenticated-orcid":false,"given":"Nor Fadhilah","family":"Kamaruzzaman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1301-5351","authenticated-orcid":false,"given":"Mohammed Dauda","family":"Goni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,10]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.3389\/bjbs.2023.11387"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.858799"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.29261\/pakvetj\/2024.285"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fvets.2021.688935"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.33899\/ijvs.2025"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S219155"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.8.2498-2507.1999"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/01652176.2013.799301"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S453025"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1515\/med-2023-0707"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.3390\/v13102102"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11030-023-10738-z"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2022.132531"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2019.125124"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu12020457"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pestbp.2022.105221"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.4c00292"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2621.2010.02267.x"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1501049112"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.3390\/v8010006"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.4103\/MJBL.MJBL_996_23"},{"key":"e_1_2_11_22_2","first-page":"25","volume-title":"Advances in Protein Chemistry and Structural Biology","author":"Kanakaveti V.","year":"2020"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.2144\/fsoa-2022-0085"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.4142\/jvs.23045"},{"key":"e_1_2_11_25_2","volume-title":"AIP Conference Proceedings","author":"Abady N. 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