{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T10:05:48Z","timestamp":1779444348245,"version":"3.53.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T00:00:00Z","timestamp":1779408000000},"content-version":"vor","delay-in-days":141,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002873","name":"Chulalongkorn University","doi-asserted-by":"publisher","award":["HEA_FF_68_163_3300_010"],"award-info":[{"award-number":["HEA_FF_68_163_3300_010"]}],"id":[{"id":"10.13039\/501100002873","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Social insects such as ants, wasps, and bees have been recognized as promising sources of actinomycetes with antimicrobial potential. In a previous study, we isolated an ant\u2010derived\n                    <jats:italic>Streptomyces<\/jats:italic>\n                    strain, LKA04, from\n                    <jats:italic>Polyrhachis laevissima<\/jats:italic>\n                    , which exhibited potent antimicrobial activity. However, the antimicrobial compound produced by this strain had not been previously characterized. Based on phenotypic and genotypic analyses, strain LKA04 was identified as\n                    <jats:italic>Streptomyces parvulus<\/jats:italic>\n                    . Bioassay\u2010guided fractionation led to the successful isolation of the purified antimicrobial compound produced by this strain. HPLC chromatographic comparison with an in\u2010house database, along with NMR and mass spectrometry analyses, identified the active compound as actinomycin D. This compound exhibited broad\u2010spectrum antimicrobial activity against gram\u2010positive bacteria, including\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    , methicillin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA),\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    ,\n                    <jats:italic>Kocuria rhizophila<\/jats:italic>\n                    ,\n                    <jats:italic>Enterococcus faecalis<\/jats:italic>\n                    , and\n                    <jats:italic>Listeria monocytogenes<\/jats:italic>\n                    . It also demonstrated activity against gram\u2010negative bacteria such as\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    and\n                    <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                    , as well as the yeasts\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    and\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    . The highest yield of actinomycin D (217\u2009mg\/L) was achieved when\n                    <jats:italic>S. parvulus<\/jats:italic>\n                    LKA04 was cultured in glutamic acid\u2013histidine\u2013fructose mineral salts broth at 30\u00b0C under shaking conditions (180\u2009rpm) for 14 days. This study highlights the potential of ant\u2010derived actinomycetes as valuable sources of antimicrobial compounds and underscores the need for continued investigation to further advance our understanding of their biosynthetic capabilities and pharmaceutical potential.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/8925797","type":"journal-article","created":{"date-parts":[[2026,5,22]],"date-time":"2026-05-22T09:26:54Z","timestamp":1779442014000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization and Production of Antimicrobial Compound by\n                    <i>Streptomyces<\/i>\n                    Isolated From the Ant\n                    <i>Polyrhachis laevissima<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"given":"Tuangrat","family":"Tunvongvinis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1362-0754","authenticated-orcid":false,"given":"Weeyawat","family":"Jaitrong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4069-4257","authenticated-orcid":false,"given":"Yudthana","family":"Samung","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6322-8622","authenticated-orcid":false,"given":"Chaisak","family":"Chansriniyom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achiraya","family":"Somphong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7149-5341","authenticated-orcid":false,"given":"Somboon","family":"Tanasupawat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1246-6700","authenticated-orcid":false,"given":"Wongsakorn","family":"Phongsopitanun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,22]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-0379-y"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2005.1"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2024.1303010"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.09.006"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.3934\/microbiol.2024005"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41429-024-00766-8"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijsem.0.001276"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10482-022-01716-w"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jnatprod.0c00772"},{"key":"e_1_2_12_10_2","doi-asserted-by":"publisher","DOI":"10.1039\/c6sc04265a"},{"key":"e_1_2_12_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-16-3-313"},{"key":"e_1_2_12_12_2","first-page":"295","volume-title":"Methods in Microbiology","author":"Williams S. 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