{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:58:46Z","timestamp":1777949926976,"version":"3.51.4"},"reference-count":82,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,3]],"date-time":"2026-05-03T00:00:00Z","timestamp":1777766400000},"content-version":"vor","delay-in-days":122,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Microbiology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Vibrio cholerae<\/jats:italic>\n                    is a gram\u2010negative bacterium found in water, particularly in brackish and estuarine environments, and causes cholera\u2014an acute disease transmitted through contaminated food or water. Biofilm formation in\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    enhances its resistance to antimicrobial agents and protection against host immune responses. Natural plant extracts have gained attention for their potential antibiofilm properties against human pathogens such as\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . This study evaluates the antibacterial and antibiofilm activities of bluestem (\n                    <jats:italic>Andropogon<\/jats:italic>\n                    spp.) extracts against pathogenic\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    strains using antibacterial assays, biofilm inhibition and eradication assays, and gene expression analysis. Two extracts, denoted as EAV1 and EAV2, primarily composed of two flavonoid C\u2010glycosides species, demonstrated significant bactericidal effects on\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    . Sub\u2010MIC levels of the extracts also inhibited the growth of biofilms. Gene expression analysis revealed downregulation of genes encoding biofilm transcriptional regulators,\n                    <jats:italic>Vibrio<\/jats:italic>\n                    polysaccharide, and biofilm matrix proteins upon EAV treatment. Results indicate a potential quorum\u2010quenching activity by targeting the transcription of biofilm regulators and biofilm matrix genes. These findings suggest that\n                    <jats:italic>Andropogon<\/jats:italic>\n                    spp. extract is a promising antibacterial and antibiofilm agent against\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    , highlighting the potential of plant species with invasive tendencies as sources of antibacterial and antibiofilm compounds against clinically important pathogens.\n                  <\/jats:p>","DOI":"10.1155\/ijm\/9982329","type":"journal-article","created":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:03:21Z","timestamp":1777871001000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antibacterial and Antibiofilm Activities of Bluestem (\n                    <i>Andropogon<\/i>\n                    spp.) Extracts against\n                    <i>Vibrio cholerae<\/i>"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9662-2511","authenticated-orcid":false,"given":"Jant Cres","family":"Caigoy","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5538-0595","authenticated-orcid":false,"given":"Tadashi","family":"Shimamoto","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7103-5698","authenticated-orcid":false,"given":"Tran Dang","family":"Xuan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6567-9902","authenticated-orcid":false,"given":"Nguyen Van","family":"Quan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,3]]},"reference":[{"key":"e_1_2_11_1_2","volume-title":"Cholera Current Topics in Infectious Disease","author":"Barua D.","year":"1992"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.3390\/microorganisms12040818"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-37660-0"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0004330"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03688.x"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.3000429"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2023.05.008"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0308707101"},{"key":"e_1_2_11_9_2","volume-title":"Technical Note on Use of Antibiotics for the Treatment of Cholera","author":"Global Task Force on Cholera Control","year":"2019"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X.JFP-17-091"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-77868-4"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-75929-2"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.3390\/metabo9110258"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sciaf.2024.e02395"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.154973"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jenvman.2020.110171"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants13060888"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2020.11.004"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26133814"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.26492\/fos7.2019-07"},{"key":"e_1_2_11_21_2","volume-title":"Native Plants for Accomack & Northampton","author":"Virginia Department of Environmental Quality","year":"2024"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/epp.12526"},{"key":"e_1_2_11_23_2","unstructured":"National Institute for Environmental Studies Andropogon virginicus inInvasive Species of Japan https:\/\/www.nies.go.jp\/biodiversity\/invasive\/DB\/detail\/81450e.html."},{"key":"e_1_2_11_24_2","unstructured":"Natural Resources Conservation Service Plant Profile for ANVI2 inPLANTS Database https:\/\/plants.usda.gov\/plant-profile\/ANVI2\/sources."},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0291365"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/265425"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2025.111162"},{"key":"e_1_2_11_28_2","volume-title":"A Field Guide to Medicinal Plants: Eastern and Central North America","author":"Foster S.","year":"2000"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants10010069"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/app12199871"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2017.1356830"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phyplu.2023.100511"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24030605"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01044-22"},{"key":"e_1_2_11_35_2","volume-title":"Performance Standards for Antimicrobial Susceptibility Testing; Twenty-Fifth Informational Supplement; M100-S25","author":"CLSI M100","year":"2026"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_1.5"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.3791\/2437"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41522-017-0026-1"},{"key":"e_1_2_11_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-020-02964-6"},{"key":"e_1_2_11_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micres.2025.128155"},{"key":"e_1_2_11_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00981-06"},{"key":"e_1_2_11_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules21101275"},{"key":"e_1_2_11_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2025.156424"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.2478\/aiht-2020-71-3396"},{"key":"e_1_2_11_45_2","doi-asserted-by":"publisher","DOI":"10.4103\/jpbs.jpbs_735_23"},{"key":"e_1_2_11_46_2","doi-asserted-by":"crossref","DOI":"10.1002\/9783527817894","volume-title":"Carbohydrate Chemistry in the Total Synthesis of Naturally Occurring Glycosides","author":"Yu B.","year":"2024"},{"key":"e_1_2_11_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2018.07.006"},{"key":"e_1_2_11_48_2","doi-asserted-by":"publisher","DOI":"10.3390\/microbiolres15040140"},{"key":"e_1_2_11_49_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-018-2321-7"},{"key":"e_1_2_11_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.sajb.2016.12.019"},{"key":"e_1_2_11_51_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.5436"},{"key":"e_1_2_11_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/biology13090676"},{"key":"e_1_2_11_53_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2021.105121"},{"key":"e_1_2_11_54_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13568-024-01776-3"},{"key":"e_1_2_11_55_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2023.1137947"},{"key":"e_1_2_11_56_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1916571117"},{"key":"e_1_2_11_57_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics12020329"},{"key":"e_1_2_11_58_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-00010-9"},{"key":"e_1_2_11_59_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foohum.2025.100533"},{"key":"e_1_2_11_60_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dky127"},{"key":"e_1_2_11_61_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11111534"},{"key":"e_1_2_11_62_2","doi-asserted-by":"publisher","DOI":"10.1093\/femsle\/fnac076"},{"key":"e_1_2_11_63_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0304126"},{"key":"e_1_2_11_64_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2013.09.006"},{"key":"e_1_2_11_65_2","doi-asserted-by":"publisher","DOI":"10.1080\/14787210.2020.1794815"},{"key":"e_1_2_11_66_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104068"},{"key":"e_1_2_11_67_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e17657"},{"key":"e_1_2_11_68_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-16507-x"},{"key":"e_1_2_11_69_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.14301"},{"key":"e_1_2_11_70_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmolb.2020.00025"},{"key":"e_1_2_11_71_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M116.770552"},{"key":"e_1_2_11_72_2","doi-asserted-by":"publisher","DOI":"10.1111\/1348-0421.12150"},{"key":"e_1_2_11_73_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2014.01.001"},{"key":"e_1_2_11_74_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.040196-0"},{"key":"e_1_2_11_75_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.188.3.1049-1059.2006"},{"key":"e_1_2_11_76_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01569-06"},{"key":"e_1_2_11_77_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086751"},{"key":"e_1_2_11_78_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1222981"},{"key":"e_1_2_11_79_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3433"},{"key":"e_1_2_11_80_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bpj.2014.10.015"},{"key":"e_1_2_11_81_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodcont.2011.09.006"},{"key":"e_1_2_11_82_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00867"}],"container-title":["International Journal of Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/ijm\/9982329","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/ijm\/9982329","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/ijm\/9982329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T05:03:25Z","timestamp":1777871005000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/ijm\/9982329"}},"subtitle":[],"editor":[{"given":"Shweta","family":"Negi","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":82,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/ijm\/9982329"],"URL":"https:\/\/doi.org\/10.1155\/ijm\/9982329","archive":["Portico"],"relation":{},"ISSN":["1687-918X","1687-9198"],"issn-type":[{"value":"1687-918X","type":"print"},{"value":"1687-9198","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-04-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-03","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9982329"}}