{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T07:16:55Z","timestamp":1783322215551,"version":"3.54.6"},"reference-count":12,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T00:00:00Z","timestamp":1783296000000},"content-version":"vor","delay-in-days":186,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Dentistry"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Vital pulp therapy (VPT) in mature permanent teeth with irreversible pulpitis represents a paradigm shift toward preserving pulp vitality. Advanced platelet\u2010rich fibrin plus (A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      ) is a bioactive scaffold capable of releasing high concentrations of growth factors; however, its concentration\u2010dependent effects on inflamed pulp\u2010derived stem cells remain unclear. This study aimed to evaluate the concentration\u2010dependent effects of A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      \u2009and identify a biologically favorable concentration range for supporting the functional activity of dental pulp stem cells isolated from teeth with irreversible pulpitis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      IP\u2010DPSCs were isolated from inflamed pulp tissues and characterized by flow cytometry and trilineage differentiation assays. A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      \u2009was prepared using the low\u2010speed centrifugation concept (LSCC). Cells were exposed to sequential dilutions of A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      \u2009extracts (100%, 50%, and 25%). Cell metabolic activity was assessed using the MTT assay. Cell migration was evaluated using a scratch assay, and wound closure rates were quantified with ImageJ software. Statistical analysis was performed using one\u2010way or two\u2010way ANOVA with appropriate post\u2010hoc tests.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Flow\u2010cytometric analysis showed high expression of MSC\u2010associated markers (CD44, CD73, and CD90) and negligible expression of hematopoietic markers, while differentiation along osteogenic, chondrogenic, and adipogenic lineages was observed following induction culture. A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      \u2009exhibited a biphasic concentration\u2010dependent effect. The 25% extract significantly enhanced cell migration, achieving a wound closure rate of 27.09\u2009\u00b1\u20094.03%, compared with 13.16\u2009\u00b1\u20094.50% in the 100% extract group (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.013). Undiluted A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      \u2009showed inhibitory effects on both migration and metabolic activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      \u2009exerted concentration\u2010dependent effects on IP\u2010DPSCs, with diluted preparations supporting greater migration and metabolic activity than undiluted extracts. These findings suggest the existence of a biologically favorable concentration range for A\u2010PRF\n                      <jats:sup>+<\/jats:sup>\n                      \u2009and provide a rationale for further investigation of concentration optimization in regenerative endodontic applications.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijod\/3582890","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T07:03:42Z","timestamp":1783321422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Effects of A\u2010PRF\n                    <sup>+<\/sup>\n                    Extract at Different Concentrations on the Metabolic Activity and Migration of Dental Pulp Stem Cells in Irreversible Pulpitis: An In Vitro Study"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1898-3167","authenticated-orcid":false,"given":"Duyen Ngoc-Minh","family":"Tran","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6981-7012","authenticated-orcid":false,"given":"Minh Duc","family":"Nguyen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ninh Hai","family":"Truong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-0931-7732","authenticated-orcid":false,"given":"Giang Thanh","family":"Pham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-0763-5321","authenticated-orcid":false,"given":"Thinh 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