{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:54:00Z","timestamp":1791010440687,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T00:00:00Z","timestamp":1790899200000},"content-version":"vor","delay-in-days":274,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100019125","name":"Sorbonne Universit\u00e9","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100019125","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100021716","name":"Universit\u00e9 de Paris","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100021716","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Dentistry"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>Nifedipine\u2010induced gingival overgrowth (NIGO) has been linked to fibrotic remodeling of the gingiva, yet whether FAM20A and Fetuin\u2010A contribute to this process is unknown. Here we ask whether their expression is altered in NIGO and, if so, whether nifedipine itself drives this change.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>\n                      We examined gingival biopsies from 18 patients under long\u2010term nifedipine treatment and 17 unaffected controls by immunofluorescence, western blotting, and RT\u2010qPCR. In parallel, human gingival fibroblasts (hGF) were exposed to 100\u2009ng\/mL nifedipine for either 48\u2009h or 7 days to test for direct drug effects on FAM20A, FAM20C, Fetuin\u2010A, alpha\u2010smooth muscle actin (\u03b1\u2010SMA), and the endocytic receptors LRP2 and CUBN. Protein expression was graded semiquantitatively, and group differences were considered significant at\n                      <jats:italic>p<\/jats:italic>\n                      \u2009 &lt; 0.05.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Both FAM20A and Fetuin\u2010A were markedly overexpressed in fibrotic NIGO gingiva. Nifedipine exposure raised FAM20A expression in hGF, yet left Fetuin\u2010A unchanged at either the transcript or protein level\u2014an apparent contradiction that prompted us to examine its receptors, LRP2 and CUBN. These were barely detectable in healthy gingiva but abundant in NIGO tissue, where Fetuin\u2010A colocalized with LRP2; nifedipine reproduced this induction of LRP2\/CUBN in cultured hGF. Together, these findings point to enhanced Fetuin\u2010A endocytosis, rather than increased synthesis, as the source of its accumulation in NIGO. TGF\u03b2 signaling was not activated under any nifedipine treatment condition tested.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>FAM20A, Fetuin\u2010A, and the LRP2\/CUBN endocytic pair emerge as central molecular players in NIGO. Our data indicate that nifedipine drives FAM20A upregulation and boosts LRP2\/CUBN\u2010dependent Fetuin\u2010A uptake through a TGF\u03b2\u2010independent route, offering a new mechanistic view of NIGO and pointing to candidate targets for future therapeutic strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijod\/9127997","type":"journal-article","created":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T06:46:51Z","timestamp":1791010011000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Nifedipine Induces FAM20A Upregulation and LRP2\/CUBN\u2010Mediated Fetuin\u2010A Accumulation in Gingival Overgrowth"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-6978-1824","authenticated-orcid":false,"given":"Isaac","family":"De la Rosa","sequence":"first","affiliation":[{"name":"Faculty of Exact and Natural Sciences Campus of San Pablo Analytical Chemistry and Biomedicine Group University of Cartagena  Cartagena de Indias 130014 Colombia  unicartagena.edu.co"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-8434-9908","authenticated-orcid":false,"given":"Amina","family":"Baker","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers Sorbonne Universit\u00e9 INSERM Universit\u00e9 de Paris Cit\u00e9 Laboratory of Oral Molecular Pathophysiology  Paris 75006 France  inserm.fr"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7474-8672","authenticated-orcid":false,"given":"Vidjeacoumary","family":"Cannaya","sequence":"additional","affiliation":[{"name":"Centre de Recherche des Cordeliers Sorbonne Universit\u00e9 INSERM Universit\u00e9 de Paris Cit\u00e9 Laboratory of Oral Molecular Pathophysiology  Paris 75006 France  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