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This study aims to elucidate the role of pivotal genes in mediating the effects of gene expression on cell senescence and TNBC progression. We employed a combination of Mendelian randomization (MR) and single\u2010cell RNA sequencing to investigate these relationships. A two\u2010sample MR approach was utilized to assess causal links between pivotal genes and TNBC. Single\u2010cell transcriptomic analysis of senescence and TNBC datasets from the Gene Expression Omnibus database revealed a significant association between the gene GZMK and increased TNBC risk. Our single\u2010cell analysis uncovered a complex network of ligand\u2013receptor interactions in GZMK\u2009+\u2009central memory CD8+ T cells (CD8_CM), which frequently interacted with macrophages, monocytes, and epithelial cells. GZMK expression was correlated with pseudotime progression, indicating its potential role in TNBC development. Enrichment analysis suggested that GZMK\u2009+\u2009CD8_CM cells play a role in enhancing immune process regulation, pointing to a broader immune response. Bulk sequencing further confirmed the presence of GZMK expression in TNBC samples. Functional experiments demonstrated that GZMK significantly influences the proliferation, migration, and invasion of TNBC cell lines\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Our findings reveal intricate interactions among immune cell composition, GZMK expression, senescence, and TNBC, highlighting GZMK as a potential molecular target for therapeutic intervention. This study advances the understanding of TNBC pathogenesis and opens new avenues for precision medicine in TNBC treatment.\n                  <\/jats:p>","DOI":"10.1155\/ijog\/4215646","type":"journal-article","created":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T12:01:17Z","timestamp":1776427277000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell Transcriptomics Reveals the Potential Role of GZMK+ CD8+ T Cells in Cell Senescence of Triple\u2010Negative Breast 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