{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T10:07:18Z","timestamp":1779271638077,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T00:00:00Z","timestamp":1779235200000},"content-version":"vor","delay-in-days":139,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007194","name":"Wenzhou Municipal Science and Technology Bureau","doi-asserted-by":"publisher","award":["Y20240241"],"award-info":[{"award-number":["Y20240241"]}],"id":[{"id":"10.13039\/501100007194","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Genomics"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Mucosal\u2010associated invariant T (MAIT) cells are innate\u2010like T lymphocytes involved in immune surveillance and tissue homeostasis. Yet, their transcriptional dynamics and functional crosstalk with macrophages at the time of myocardial injury are incompletely defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We used two publicly available single\u2010cell RNA sequencing (scRNA\u2010seq) datasets from a murine ischemia\u2013reperfusion myocardial injury model (GSM8252253 and GSM8252254). Following quality control and clustering, immune cell composition, cytokine expression patterns, and macrophage polarization states were systematically characterized. Concurrently, in vitro coculture experiments validated the MR1\u2010dependent regulatory effects of 5\u2010OP\u2010RU\u2013activated MAIT cells on macrophage cytokine expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Single\u2010cell analysis showed significant immune remodeling on Day 7 post\u2010injury, with a relative expansion of B cells and depletion of macrophages compared to controls. UMAP\u2010based feature mapping revealed that expression of Tnf was significantly restricted to myeloid compartments (especially monocytes), whereas Il10 exhibited a diffuse pattern across immune subsets. MAIT cells exhibited increased expression of signature genes such as ZBTB16 and KLRB1 consistent with activation\u2010associated transcriptional reprogramming after damage. Macrophage populations exhibited heterogeneous polarization states, with limited TNF\u2010\u03b1\/IL\u201010 coexpression and overall low Il10 abundance in vivo. Functional validation showed that 5\u2010OP\u2010RU\u2013activated MAIT cells significantly suppressed TNF\u2010\u03b1 while enhancing IL\u201010 expression in macrophages, and these effects were largely reversed by MR1 blockade.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our findings reveal dynamic immune cell remodeling and transcriptional adaptation of MAIT cells during myocardial injury. The predominance of Tnf\u2010driven myeloid signaling and the MR1\u2010dependent anti\u2010inflammatory modulation of macrophages by activated MAIT cells highlight a potential regulatory axis in cardiac inflammation. These results provide mechanistic insight into MAIT\u2013macrophage interactions and suggest potential immunomodulatory targets for myocardial injury intervention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijog\/5460709","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T09:25:04Z","timestamp":1779269104000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Single\u2010Cell RNA Sequencing Reveals Dynamic Expression Patterns of MAIT Cells and Macrophage Subpopulations in Myocardial Injury Model"],"prefix":"10.1155","volume":"2026","author":[{"given":"Haofei","family":"Kang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junjie","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renjie","family":"Ruan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yudan","family":"Wen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bingru","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meixiang","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1042-2009","authenticated-orcid":false,"given":"Yongguang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-5645-7654","authenticated-orcid":false,"given":"Yunrui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,20]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.664457"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-08-303339"},{"key":"e_1_2_11_3_2","article-title":"The Immune Modulating Properties of Mucosal-Associated Invariant T Cells","volume":"11","author":"Cosedis N. 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O.","year":"2008","journal-title":"Journal of Leukocyte Biology"},{"key":"e_1_2_11_9_2","first-page":"289","article-title":"Macrophage Polarization in Inflammatory Diseases","volume":"11","author":"Shapouri-Moghaddam A.","year":"2018","journal-title":"Journal of Inflammation Research"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2004.09.015"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-020-0359-y"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.7554\/elife.43882"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/smtd.202100752"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1161\/jaha.122.027228"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3565"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0059-x"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-019-0715-4"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1161\/circresaha.116.303577"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw2719"},{"key":"e_1_2_11_20_2","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/bs.ai.2015.03.003","article-title":"Activation and Function of iNKT and MAIT Cells","volume":"127","author":"Chandra S.","year":"2015","journal-title":"Advances in Immunology"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.06.008"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci45444"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01478"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.4ta0815-391rr"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aax6624"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-019-0191-y"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.07.039"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20171739"}],"container-title":["International Journal of Genomics"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/ijog\/5460709","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/ijog\/5460709","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/ijog\/5460709","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T09:25:09Z","timestamp":1779269109000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/ijog\/5460709"}},"subtitle":[],"editor":[{"given":"Zhengrui","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/ijog\/5460709"],"URL":"https:\/\/doi.org\/10.1155\/ijog\/5460709","archive":["Portico"],"relation":{},"ISSN":["2314-436X","2314-4378"],"issn-type":[{"value":"2314-436X","type":"print"},{"value":"2314-4378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-11-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-20","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"5460709"}}