{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T09:01:06Z","timestamp":1780218066504,"version":"3.54.0"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T00:00:00Z","timestamp":1780185600000},"content-version":"vor","delay-in-days":150,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Genomics"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Acute myocardial infarction (AMI) represents a life\u2010threatening cardiovascular emergency characterized by myocardial necrosis resulting from prolonged ischemia.\n                      <jats:italic>Erigeron breviscapus<\/jats:italic>\n                      (Vant.) Hand.\u2010Mazz. (EB), a traditional Chinese medicinal herb, has demonstrated cardioprotective properties in clinical applications. This investigation aims to establish a predictive computational framework for investigating the molecular mechanisms through which EB exerts therapeutic effects in AMI employing integrated network pharmacology and single\u2010cell transcriptomic strategies.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Active pharmaceutical constituents of EB and their corresponding molecular targets were systematically retrieved from TCMSP and SwissTargetPrediction databases. AMI\u2010associated genes were compiled from GeneCards, OMIM, and DisGeNET repositories. Protein\u2013protein interaction (PPI) networks were constructed utilizing STRING database, with core targets identified through topological analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to characterize biological functions. Single\u2010cell RNA sequencing (scRNA\u2010seq) data from myocardial infarction specimens were processed using 10\u00d7 Genomics platform. Comprehensive analytical workflows included Seurat\u2010based clustering algorithms, UMAP dimensionality reduction, differential expression profiling, functional enrichment characterization, and Monocle 3\u2010mediated pseudotime trajectory reconstruction. Experimental validations encompassed murine AMI models, cellular senescence assays, and molecular interaction studies.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Network pharmacology analysis identified 45 bioactive compounds from EB corresponding to 286 potential therapeutic targets. Cross\u2010referencing with AMI\u2010related genes yielded 127 overlapping targets, suggesting multitarget pharmacological interventions. PPI network topology analysis revealed hub genes including VEGFA, AKT1, TNF, IL6, and PTGS2, indicating predicted involvement in angiogenesis, inflammation, and apoptotic pathways. KEGG enrichment demonstrated significant associations with PI3K\u2010AKT, MAPK, and TNF signaling cascades. Single\u2010cell transcriptomic profiling successfully delineated heterogeneous cardiac cell populations comprising cardiomyocytes, cardiac fibroblasts, endothelial cells, macrophages, and T lymphocytes. Differential expression analysis revealed substantial transcriptional reprogramming in post\u2010infarction myocardium, particularly within macrophage and fibroblast populations. Pseudotime trajectory analysis elucidated dynamic cellular state transitions during myocardial repair processes. Integration of network pharmacology predictions with scRNA\u2010seq data demonstrated that predicted EB target genes exhibited preferential expression in inflammation\u2010associated cell types. Functional enrichment of differentially expressed genes highlighted extracellular matrix reorganization, inflammatory response modulation, and neovascularization as principal biological processes potentially targeted by EB intervention.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Through systematic integration of network pharmacology and single\u2010cell transcriptomics, this investigation established a predictive computational framework characterizing the multicomponent, multitarget, multipathway mechanisms underlying EB\u2010mediated cardioprotection in AMI. The findings provide computational evidence and hypothesis\u2010generating insights supporting EB therapeutic applications and establish a foundation for future experimental validation and precision medicine approaches in AMI management.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijog\/7715750","type":"journal-article","created":{"date-parts":[[2026,5,31]],"date-time":"2026-05-31T08:23:56Z","timestamp":1780215836000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Network Pharmacology and Single\u2010Cell Transcriptomic Investigation of Molecular Mechanisms Underlying\n                    <i>Erigeron breviscapus<\/i>\n                    Treatment in Acute Myocardial Infarction"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4652-9256","authenticated-orcid":false,"given":"Lili","family":"Yan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-0693-7481","authenticated-orcid":false,"given":"Jun","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9522-4102","authenticated-orcid":false,"given":"Qinglong","family":"Xie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9175-331X","authenticated-orcid":false,"given":"Lei","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9641-6168","authenticated-orcid":false,"given":"Yaqin","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,31]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-023-01652-9"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/07853890.2021.2002926"},{"key":"e_1_2_12_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/echo.15245"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.4244\/eij-d-21-00904"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1329969"},{"key":"e_1_2_12_6_2","doi-asserted-by":"publisher","DOI":"10.4103\/ijabmr.ijabmr_415_23"},{"key":"e_1_2_12_7_2","article-title":"Recent Advances of Traditional Chinese Medicine Against Cardiovascular Disease: Overview and Potential Mechanisms","volume":"15","author":"Dai J.","year":"2024","journal-title":"Frontiers in Endocrinology"},{"key":"e_1_2_12_8_2","article-title":"Cellular Heterogeneity of the Heart","volume":"9","author":"Anto M. 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