{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,9]],"date-time":"2026-03-09T03:06:02Z","timestamp":1773025562595,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":350,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["International Journal of Genomics"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Metastasis is a major adverse prognostic factors of colon cancer. Aspirin chemoprophylaxis may improve outcomes for metastatic colon cancer patients. This study aimed to determine the impact of metastasis\u2010related molecular subtypes on prognosis and aspirin chemoprophylaxis benefit.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We obtained differentially expressed metastasis\u2010related genes in cancer and normal tissues. A weighted gene co\u2010expression network (WGCNA) was constructed by differentially expressed genes. Lasso\u2010Cox regression identified key prognostic genes within relevant modules, establishing a risk score model. Transcription factors regulating module genes were explored. Aspirin\u2010interacting genes were identified using the Comparative Toxicogenomics Database (CTD) and validated via cellular experiments.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      WGCNA analysis of 2062 metastasis\u2010related genes revealed significant correlations between blue\/yellow modules and colon cancer. A risk score model based on blue module genes predicted overall survival and 1\u2010, 3\u2010, and 5\u2010year survival rates. Transcription factor analysis implicated the E2F family in blue module regulation and NF\n                      <jats:italic>\u03ba<\/jats:italic>\n                      B1\/STAT3 in yellow module regulation. CTD analysis showed persistent upregulation of NOX4, CXCL8, CXCL5, GDF15, and MMP13 post\u2010aspirin treatment. Cellular experiments confirmed aspirin downregulated metastasis\u2010related genes (E2F1, CCNE1, VEGFA, MMP3) in colon cancer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We developed a validated metastasis gene predictive model. Colon cancer patients with upregulated NOX4, CXCL8, CXCL5, GDF15, or MMP13 may not benefit from aspirin chemoprophylaxis. Conversely, patients showing aspirin\u2010induced downregulation of E2F1, CCNE1, VEGFA, and MMP3 may derive chemoprophylactic benefit.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/ijog\/8412254","type":"journal-article","created":{"date-parts":[[2025,12,18]],"date-time":"2025-12-18T06:51:07Z","timestamp":1766040667000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Risk Prediction of Colon Cancer Metastasis and Bioinformatics Analysis of Aspirin Treatment"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9032-3772","authenticated-orcid":false,"given":"Jing","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinyue","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4656-1500","authenticated-orcid":false,"given":"Jingjing","family":"Shao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-3796-0384","authenticated-orcid":false,"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9793-1067","authenticated-orcid":false,"given":"Jiawei","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jncc.2024.05.001"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21338"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.11.037"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2019.06.047"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.54.3.150"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djab008"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40271-021-00506-2"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1094"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp101"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt019"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003731"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09234-6"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkaa891"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41420-024-02223-9"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06226-9"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1203543"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_13_18_2","first-page":"1737","article-title":"Invade or Proliferate? 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