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Currently, there are relatively few established quantitative methods available for analyzing flavonols and their glycosides, and the research regarding their transformation patterns under simulated gastrointestinal conditions is still limited. In this study, network pharmacology was employed to predict the active compounds and targets of YMOS associated with cardiovascular and cerebrovascular protection. A comprehensive network integrating compounds, targets, bioactivities, and CCVDs was meticulously constructed for 10 flavonol glycosides and their aglycones. It revealed that these compounds exert their blood circulation\u2013promoting and blood stasis\u2013eliminating effects by modulating 20 key targets, highlighting their therapeutic potential in CCVDs treatment. Furthermore, we quantitatively analyzed the 10 targeted flavonols and their glycosides in YMOS using ultra\u2010performance liquid chromatography (UPLC), demonstrating consistent flavonoid levels across all tested samples. This method was also employed to investigate biotransformation rules between flavonol glycosides and their aglycones in vitro. Notably, it was observed that flavonol glycosides undergo a deglycosylation reaction in the rat\u2019s intestinal microbial culture medium, which was witnessed in a relatively stable manner under the simulated artificial gastrointestinal fluid. These findings provide important scientific insights into the mechanism of action and the quality control of YMOS.<\/jats:p>","DOI":"10.1155\/jamc\/1287338","type":"journal-article","created":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T14:15:45Z","timestamp":1782915345000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative Analysis of Flavonols and Their Glycosides and Gastrointestinal Transformation of Flavonol Glycosides in Yinxing Mihuan Oral Solution"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-9621-6958","authenticated-orcid":false,"given":"Yun","family":"Lyu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-9145-5101","authenticated-orcid":false,"given":"Wenqian","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-0071-5989","authenticated-orcid":false,"given":"Kejing","family":"Niu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoqing","family":"He","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1590-0198","authenticated-orcid":false,"given":"Lulu","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5459-1868","authenticated-orcid":false,"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3222-7608","authenticated-orcid":false,"given":"Huijuan","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,7,1]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19061734"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-023-04027-3"},{"key":"e_1_2_11_3_2","volume-title":"Pharmacopoeia of the People\u2032s Republic of China","author":"Chinese Pharmacopoeia Commission","year":"2020"},{"key":"e_1_2_11_4_2","first-page":"46","article-title":"Recent Research on Chemical Composition and Pharmacological Function of Armillaria Mellea","author":"Yuan Y.","year":"2008","journal-title":"Academic Periodical of Farm Products Processing"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.03.107"},{"key":"e_1_2_11_6_2","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1007\/BF02836532","article-title":"Progress in Researches on the Pharmaceutical Mechanism and Clinical Application of Ginkgo biloba Extract on Various Kinds of Diseases","volume":"12","author":"Wang G. 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