{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T08:15:00Z","timestamp":1788077700846,"version":"build-2784847793"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":241,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Four distinct, chemically asynchronous spectrophotometric pathways each tapping into a different reactive node of the polyfunctional doxycycline hyclate (DOX) framework were planned to bypass the limitations of single mechanism assays. It works amazingly well. By exploiting the drug\u2019s inherent structural mutability, we developed a multipronged quantification matrix. Take the drug\u2019s reducing capacity, for instance. In Method A, this specific driving force reduces Fe (III) in the presence of potassium ferricyanide, yielding a highly conjugated Turnbull\u2019s blue complex characterized by a distinct bathochromic shift (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    783.5\u2009nm). Method B switches components entirely. It forces an alkaline electrophilic coupling with Fast Red B salt to lock down a resilient red azo chromophore (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    498\u2009nm). Method C determines for selective oxidation using N\u2010bromosuccinimide (NBS) in a strongly acidic environment to generate a stable yellow derivative (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    383.5\u2009nm) (a sequence highly dependent on strict kinetic control). Method D abandons covalent modification altogether, relying instead on the generation of a lipophilic ion\u2010pair associate with bromothymol blue (BTB) at pH 3.0, which partition readily into chloroform (\n                    <jats:italic>\u03bb<\/jats:italic>\n                    <jats:sub>max<\/jats:sub>\n                    409.5\u2009nm). Linearity spans diverse concentration intervals: 0.5\u20134, 5\u201360, 20\u2013140, and 5\u201335\u2009\u03bcg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    for protocols A through D. Sensitivity peaks at a remarkably low LOD of 0.16\u2009\u03bcg\u2009mL\n                    <jats:sup>\u22121<\/jats:sup>\n                    . Rigorous compliance with ICH validation protocols confirmed tight fidelity regarding accuracy and reproducibility. When applied to real\u2010world commercial capsules, the matrix remained entirely unaffected by matrix excipients. This makes it an incredibly cheap, solvent\u2010lean alternative for high\u2010throughput benchtop quality control.\n                  <\/jats:p>","DOI":"10.1155\/jamc\/2780011","type":"journal-article","created":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T07:55:15Z","timestamp":1788076515000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Harnessing the Structural Variability of Doxycycline Hyclate: Mechanistic Insights and Comparative Assessment of Four Chemically Diverse Spectrophotometric Pathways"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-0978-469X","authenticated-orcid":false,"given":"Mona A. 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N.","year":"2022","journal-title":"Asian Journal of Pharmaceutical Analysis"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2020.09.045"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/slct.202300183"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.21123\/2411-7986.5016"},{"key":"e_1_2_10_16_2","doi-asserted-by":"publisher","DOI":"10.1134\/s1061934811050157"},{"key":"e_1_2_10_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/23080477.2025.2579256"},{"key":"e_1_2_10_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/sscp.202300173"},{"key":"e_1_2_10_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5626"},{"key":"e_1_2_10_20_2","first-page":"234","article-title":"New Methodology Colorimetric-SFI Analysis for the Determination of Doxycycline Hyclate in Pharmaceutical Preparations, Ibn Al-Haitham J. Pure","volume":"38","author":"Magid N.","year":"2025","journal-title":"Applied Sciences"},{"key":"e_1_2_10_21_2","first-page":"115","article-title":"High Performance Thin Layer Chromatography (HPTLC) Method Development and Validation for Determination of Doxycycline Hyclate in Capsule and Tablet Formulations","author":"Kumssa L.","year":"2021","journal-title":"Journal of Planar Chromatography\u2014Modern TLC"},{"key":"e_1_2_10_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cclet.2019.04.026"},{"key":"e_1_2_10_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s44211-023-00395-5"},{"key":"e_1_2_10_24_2","doi-asserted-by":"crossref","DOI":"10.1016\/j.heliyon.2023.e15223","article-title":"Green Ecofriendly Electrochemical Sensing Platform for the Sensitive Determination of Doxycycline","volume":"9","author":"Asran A. 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