{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:04:10Z","timestamp":1770879850596,"version":"3.50.1"},"reference-count":61,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":41,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100019065","name":"Tianjin Science and Technology Program","doi-asserted-by":"publisher","award":["24ZYJDSS00300"],"award-info":[{"award-number":["24ZYJDSS00300"]}],"id":[{"id":"10.13039\/501100019065","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>Fuganlin oral liquid (FOL) has been clinically employed for the treatment of pediatric qi deficiency colds, manifesting symptoms such as fever, cough, asthma, and sore throat. However, the chemical composition and bioactive components of FOL have not been clearly elucidated. In this study, a comprehensive qualitative analysis of FOL was conducted utilizing ultra\u2010high\u2010performance liquid chromatography coupled with quadrupole time\u2010of\u2010flight mass spectrometry in conjunction with network pharmacology. A total of 124 chemical components were tentatively characterized, comprising flavonoids, phenolic acids, saponins, coumarins, and others. Among these, 43 compounds were unequivocally identified by comparison with authentic reference standards. Furthermore, network pharmacology analysis revealed that the ingredients of FOL exhibited anti\u2010inflammatory properties and demonstrated potential efficacy in relieving cough and asthma associated with respiratory tract infections. Collectively, this study provides the first comprehensive characterization of the chemical composition in FOL and explores the potential pharmacological mechanisms of its bioactive constituents, thereby providing scientific support for quality control standards and clinical applications.<\/jats:p>","DOI":"10.1155\/jamc\/3075224","type":"journal-article","created":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T05:43:39Z","timestamp":1770875019000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Characterization and Mechanism Prediction of Active Components in Fuganlin Oral Liquid for Respiratory Tract Infections Using UPLC\u2013Q\u2010TOF\u2013MS and Network Pharmacology"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-5306-185X","authenticated-orcid":false,"given":"Mengyue","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4843-3109","authenticated-orcid":false,"given":"Feng","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7841-9673","authenticated-orcid":false,"given":"Mingxuan","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-0909-0404","authenticated-orcid":false,"given":"Zhishan","family":"Ye","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2770-2152","authenticated-orcid":false,"given":"Ying","family":"Cui","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0956-6046","authenticated-orcid":false,"given":"Yuefei","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5459-1868","authenticated-orcid":false,"given":"Jing","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2212-7777","authenticated-orcid":false,"given":"Xin","family":"Chai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,11]]},"reference":[{"key":"e_1_2_8_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lanmic.2024.100967"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00203-024-04107-z"},{"key":"e_1_2_8_3_2","first-page":"168","article-title":"Clinical Effects of Fuganlin Oral Liquid on Recurrent Respiratory Tract Infections","volume":"24","author":"Tang Y.","year":"2019","journal-title":"Lingnan Journal of Emergency Medicine"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2024.1431862"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13020-024-01056-z"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.118"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.19540\/j.cnki.cjcmm.20200915.301"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1750"},{"key":"e_1_2_8_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2023.115456"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.13748\/j.cnki.issn1007-7693.2022.15.011"},{"key":"e_1_2_8_11_2","first-page":"3438","article-title":"Spatial Distribution of Coumarins in Angelica pubescens Fresh Roots by MALDI-MSI","volume":"54","author":"Li Q.","year":"2023","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_12_2","volume-title":"Studies on Chemical Constituents and the Related Application of Lentinus edodes","author":"Zhang N.","year":"2013"},{"key":"e_1_2_8_13_2","first-page":"3173","article-title":"The Mechanism of Isatidis Radix in the Prevention of Influenza and COVID-19 by HPLC-Q-TOF-MS Combined With Network Pharmacology","volume":"57","author":"Wang X. Q.","year":"2023","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.13748\/j.cnki.issn1007-7693.20222143"},{"key":"e_1_2_8_15_2","volume-title":"Analysis of Chemical Constituents Differences Between Fritillaria cirrhosa D. Don and Fritillaria thunbergii and Its Metabolomics Based on UPLC-Q-TOF-MS\/MS Technology","author":"Ji S.","year":"2022"},{"key":"e_1_2_8_16_2","doi-asserted-by":"publisher","DOI":"10.14148\/j.issn.1672-0482.2022.1143"},{"key":"e_1_2_8_17_2","first-page":"470","article-title":"Analysis of Chemical Constituents of Rhododendron dauricum L. Based on Liquid Chromatography-Mass Spectrometry","volume":"30","author":"Fan X. Y.","year":"2023","journal-title":"Chinese Journal of Traditional Medical Science and Technology"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.19405\/j.cnki.issn1000-1492.2015.10.021"},{"key":"e_1_2_8_19_2","first-page":"283","article-title":"Studies on Chemical Constituents of Bidens bipinnata","volume":"25","author":"Yang X. W.","year":"2009","journal-title":"Medical Journal of Chinese People\u2019s Liberation Army"},{"key":"e_1_2_8_20_2","volume-title":"Studies on the Chemical Constituents and Biological Activities of Bidens parviflora Willd.","author":"Li C. H.","year":"2020"},{"key":"e_1_2_8_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.202100330"},{"key":"e_1_2_8_22_2","volume-title":"Study on Chemical Composition Analysis and Metabolic Material Basis In Vivo of Modified Suanzaoren Decoction","author":"Ren H. D.","year":"2020"},{"key":"e_1_2_8_23_2","volume-title":"Qualitative and Quantitative Characterization of Chemomes in Sini San Using Liquid Chromatography-Mass Spectrometry Method","author":"Xu X.","year":"2019"},{"key":"e_1_2_8_24_2","first-page":"453","article-title":"Chemical Constituents of Crepis phoenix","volume":"54","author":"Peng B.","year":"2023","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_25_2","doi-asserted-by":"publisher","DOI":"10.16781\/j.0258-879x.2006.08.021"},{"key":"e_1_2_8_26_2","first-page":"765","article-title":"Studies on the Flavonoids and Antilipid Peroxidantion of Bidens bipinnata","volume":"18","author":"Jiang T.","year":"2006","journal-title":"Natural Product Research and Development"},{"key":"e_1_2_8_27_2","first-page":"270","article-title":"Analysis on Chemical Principles of Huoxiangzhengqi Solution by HPLC-MS","volume":"29","author":"Lu J. J.","year":"2006","journal-title":"Journal of Beijing University of Traditional Chinese Medicine"},{"key":"e_1_2_8_28_2","first-page":"809","article-title":"Comprehensive Screening of Multi-Components in Huangqi Injection by Ultra-Performance Liquid Chromatography Coupled With Quadrupole-Time-of-Flight Mass Spectrometry","volume":"14","author":"Yu H.","year":"2019","journal-title":"World Chinese Medicine"},{"key":"e_1_2_8_29_2","first-page":"1436","article-title":"Chemical Constituents in the Ethyl Acetate Fraction From Whole Herb of Bidens pilosa var. Radiata Schult. Bip. in Fujian Province","volume":"23","author":"Wan Z. X.","year":"2020","journal-title":"China Pharmacist"},{"key":"e_1_2_8_30_2","doi-asserted-by":"crossref","first-page":"518","DOI":"10.5246\/jcps.2011.05.066","article-title":"Flavonoids From the Herb of Bidens pilosa L.","volume":"20","author":"Tian X.","year":"2011","journal-title":"Journal of Chinese Pharmaceutical Sciences"},{"key":"e_1_2_8_31_2","first-page":"7339","article-title":"Qualitative and Quantitative Study on Chemical Constituents in Scutellariae Radix Decoction","volume":"53","author":"Gao W. Y.","year":"2022","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_32_2","first-page":"12","article-title":"Comparison of the Difference of Chemical Compositions in Astragali Radix Decoction Pieces From Different Origins by UHPLC-Q-Orbitrap-MS","volume":"39","author":"Liu Q.","year":"2024","journal-title":"Northwest Pharmaceutical Journal"},{"key":"e_1_2_8_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2004.08.010"},{"key":"e_1_2_8_34_2","first-page":"964","article-title":"Rapid Identification of Chemical Constituents in Huangqi Jianzhong Tang by UHPLC Coupled With Hybrid Quadrupole-Orbitrap MS","volume":"52","author":"Hu Y. H.","year":"2017","journal-title":"Acta Pharmaceutica Sinica"},{"key":"e_1_2_8_35_2","volume-title":"Study on Chemical Composition and Anti-Myocardial Ischemia Effect of Fresh Berries of Panax quinquefolium L.","author":"Li L.","year":"2022"},{"key":"e_1_2_8_36_2","first-page":"2061","article-title":"Analysis on Chemical Constituents From Glycyrrhizae Radix et Rhizoma by HPLC-Q-TOF-MS","volume":"47","author":"Zhao Y. M.","year":"2016","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_37_2","volume-title":"Chemical Components Analysis of Radix Peucedani by HPLC-MS and Library Searching Technologies","author":"Jiang X. J.","year":"2013"},{"key":"e_1_2_8_38_2","first-page":"500","article-title":"Analysis of Chemical Constituents in Artemisia argyi (Nanyang) by UPLC-Q-TOF-MS\/MS","volume":"38","author":"Wang Y. Q.","year":"2018","journal-title":"Chinese Journal of Hospital Pharmacy"},{"key":"e_1_2_8_39_2","volume-title":"Application of LC-MS in the Discovery of Q-Markers of Glycyrrhiza uralensis and Paeonia lactiflora With Different Processing Degrees and Metabolic Transformation of Ginsenosides by Gut Microbiota","author":"Yao L. L.","year":"2021"},{"key":"e_1_2_8_40_2","first-page":"1277","article-title":"Effects of Different Harvest Seasons on Anthraquinone Contents of Polygoni Multiflori Radix Based on UPLC-MS\/MS","volume":"19","author":"Li Y. Y.","year":"2022","journal-title":"Chinese Journal of Pharmacovigilance"},{"key":"e_1_2_8_41_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules201119712"},{"key":"e_1_2_8_42_2","first-page":"6686","article-title":"Composition Analysis and Fingerprint Establishment of Tongmai Jiangtang Capsule Based on HPLC-Q-Exactive-MS and HPLC","volume":"53","author":"Wu Y.","year":"2022","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_43_2","first-page":"2965","article-title":"Simultaneous Determination of Eight Constituents in Shaoyao Gancao Decoction by UPLC-MS\/MS","volume":"43","author":"Yang R.","year":"2021","journal-title":"Chinese Traditional Patent Medicine"},{"key":"e_1_2_8_44_2","volume-title":"Study on Chemical Constituents of Panax quinquefolium L. From Different Producing Regions at Home and Abroad","author":"Jiao Y. F.","year":"2021"},{"key":"e_1_2_8_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmu155"},{"key":"e_1_2_8_46_2","first-page":"627","article-title":"Study on the Chemical Constituents of Bidens pilosa L","volume":"3","author":"Zhao Z. Y.","year":"2022","journal-title":"Journal of Yunnan Nationalities University"},{"key":"e_1_2_8_47_2","volume-title":"Anti-Inflammatory Effect of Total Flavones of Bidens bipinnata L (TFB) and the Possible Mechanisms","author":"Lin M. Y.","year":"2013"},{"key":"e_1_2_8_48_2","doi-asserted-by":"publisher","DOI":"10.15889\/j.issn.1002-1302.2018.21.056"},{"key":"e_1_2_8_49_2","first-page":"1449","article-title":"Effects and Mechanism of Quercetin on Blood Pressure, Intestinal Flora and Ventricular Remodeling in Spontaneously Hypertensive Rats","volume":"32","author":"Zhou X.","year":"2020","journal-title":"Natural Product Research and Development"},{"key":"e_1_2_8_50_2","doi-asserted-by":"publisher","DOI":"10.13194\/j.issn.1673-842x.2024.01.020"},{"key":"e_1_2_8_51_2","doi-asserted-by":"publisher","DOI":"10.3390\/md21100535"},{"key":"e_1_2_8_52_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules26216712"},{"key":"e_1_2_8_53_2","first-page":"292","article-title":"Progress in Research on the Antithrombotic Mechanism of Food-Derived Organic Acids and Their Derivatives","volume":"43","author":"Ning Y. W.","year":"2022","journal-title":"Food Science"},{"key":"e_1_2_8_54_2","first-page":"5462","article-title":"Research Progress on Pharmacological Action and Toxicity Mechanism of Coumarins","volume":"54","author":"Dong Y.","year":"2023","journal-title":"Chinese Traditional and Herbal Drugs"},{"key":"e_1_2_8_55_2","doi-asserted-by":"publisher","DOI":"10.1002\/ardp.201900380"},{"key":"e_1_2_8_56_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioorg.2020.104163"},{"key":"e_1_2_8_57_2","doi-asserted-by":"publisher","DOI":"10.3390\/nu15183866"},{"key":"e_1_2_8_58_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.78.8.4289-4298.2004"},{"key":"e_1_2_8_59_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.41666"},{"key":"e_1_2_8_60_2","first-page":"1","article-title":"Effect of Maxing Shigantang on Airway Injury and EGFR Expression in Asthma Model Rats","volume":"28","author":"Lei T.","year":"2022","journal-title":"Chinese Journal of Experimental Traditional Medical Formulae"},{"key":"e_1_2_8_61_2","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-021-00484-5"}],"container-title":["Journal of Analytical Methods in Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jamc\/3075224","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/jamc\/3075224","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jamc\/3075224","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T06:16:02Z","timestamp":1770876962000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/jamc\/3075224"}},"subtitle":[],"editor":[{"given":"Rongda","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":61,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/jamc\/3075224"],"URL":"https:\/\/doi.org\/10.1155\/jamc\/3075224","archive":["Portico"],"relation":{},"ISSN":["2090-8865","2090-8873"],"issn-type":[{"value":"2090-8865","type":"print"},{"value":"2090-8873","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-01-19","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-02-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3075224"}}