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Its dysregulation is closely associated with various neurological disorders such as depression, Parkinson\u2019s disease, and epilepsy. However, conventional detection techniques such as microdialysis coupled with liquid chromatography are constrained by limited spatiotemporal resolution and inherent invasiveness, largely due to NE\u2019s low concentration, rapid metabolism, and complex distribution in vivo. These limitations make real\u2010time, in situ dynamic monitoring particularly challenging. In recent years, fluorescence and bioluminescence probe technologies, founded on molecular recognition or self\u2010assembly strategies, have enabled notable advances in the in situ detection and imaging of NE, owing to their superior spatiotemporal resolution, favorable biocompatibility, and versatile design. This review summarizes several representative studies in the field, covering probe design principles, response mechanisms, analytical performance, and biological applications. It also highlights emerging frontiers such as subcellular localization, in vivo dynamic imaging, millisecond\u2010scale monitoring, multitarget cooperative recognition, and integrated diagnosis and therapy. These technologies have deepened our understanding of the spatiotemporal dynamics of NE in physiological and pathological processes, while also providing powerful tools for mechanistic research, early diagnosis, and precise treatment of neuropsychiatric disorders.<\/jats:p>","DOI":"10.1155\/jamc\/5515088","type":"journal-article","created":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T12:42:48Z","timestamp":1792240968000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Frontiers in Neurotransmitter Probes: Innovations and Perspectives in Norepinephrine Detection Technology"],"prefix":"10.1155","volume":"2026","author":[{"given":"Rufei","family":"Ma","sequence":"first","affiliation":[{"name":"School of Physical Education and International Equestrian Academy Wuhan Business University  Wuhan 430056 China  wbu.edu.cn"},{"name":"Research Center for Modern Equine Industry Development Wuhan Business University  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