{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T23:27:07Z","timestamp":1773790027123,"version":"3.50.1"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T00:00:00Z","timestamp":1765238400000},"content-version":"vor","delay-in-days":342,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100012046","name":"Vietnam Academy of Science and Technology","doi-asserted-by":"publisher","award":["NCXS 01.02\/23-25"],"award-info":[{"award-number":["NCXS 01.02\/23-25"]}],"id":[{"id":"10.13039\/100012046","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:italic>Pandanus tectorius<\/jats:italic>\n                    fruits are a promising but underutilized source of bioactive constituents. We optimized extraction conditions for phenolic\u2010 and saponin\u2010enriched fractions using Box\u2013Behnken\/response surface methodology across ethanol concentration, temperature, solvent\u2010to\u2010material ratio, and time and then evaluated antioxidant and anti\u2010inflammatory activities. Total phenolic content (TPC; Folin\u2013Ciocalteu, 760\u2009nm) and total saponin content (TSC; vanillin\u2013sulfuric acid, 560\u2009nm) served as responses for model fitting (\n                    <jats:italic>R<\/jats:italic>\n                    <jats:sup>2<\/jats:sup>\n                    \u2009&gt;\u20090.96), validation, and multiresponse optimization that yielded seven distinct optimums targeting different extract profiles. Phenolic\u2010rich extracts showed potent DPPH and hydroxyl radical scavenging, whereas saponin\u2010rich extracts more strongly inhibited LPS\u2010induced nitric oxide in RAW 264.7 cells; excessive saponin enrichment, however, coincided with cytotoxicity. These results demonstrate that tuned extraction can deliver purpose\u2010built extracts for antioxidant or anti\u2010inflammatory applications, supporting the valorization of\n                    <jats:italic>P. tectorius<\/jats:italic>\n                    as a natural source for functional and nutraceutical ingredients.\n                  <\/jats:p>","DOI":"10.1155\/jamc\/5539843","type":"journal-article","created":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:06:03Z","timestamp":1765325163000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Optimization of Phenolic\u2010 and Saponin\u2010Enriched Extraction From\n                    <i>Pandanus tectorius<\/i>\n                    Fruit Using Box\u2013Behnken Design and Evaluation of Their Bioactivities"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9227-6992","authenticated-orcid":false,"given":"Do","family":"Hoang Giang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4470-8321","authenticated-orcid":false,"given":"Nguyen","family":"Hai Dang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1726-5673","authenticated-orcid":false,"given":"Tran","family":"Thi Thu Phuong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7628-4674","authenticated-orcid":false,"given":"Le","family":"Thanh Huong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3277-3415","authenticated-orcid":false,"given":"Nguyen","family":"Thu Uyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7938-5681","authenticated-orcid":false,"given":"Nguyen","family":"Thi Luyen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7241-5434","authenticated-orcid":false,"given":"Nguyen","family":"Thi Thu Thuy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1274-8759","authenticated-orcid":false,"given":"Hoang Le","family":"Tuan Anh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7676-3755","authenticated-orcid":false,"given":"Nguyen","family":"Ngoc Tung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3645-5157","authenticated-orcid":false,"given":"Nguyen","family":"Tien Dat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,9]]},"reference":[{"key":"e_1_2_10_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ympev.2014.11.002"},{"key":"e_1_2_10_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bse.2011.09.001"},{"key":"e_1_2_10_3_2","first-page":"359","article-title":"Two New Phenolic Compounds from the Fruits of Pandanus tectorius Soland","volume":"7","author":"Zhang X.","year":"2013","journal-title":"Records of Natural Products"},{"key":"e_1_2_10_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molstruc.2025.142322"},{"key":"e_1_2_10_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jnutbio.2013.12.002"},{"key":"e_1_2_10_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11418-007-0218-8"},{"key":"e_1_2_10_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phytol.2020.04.008"},{"key":"e_1_2_10_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arabjc.2015.11.003"},{"key":"e_1_2_10_9_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2014.1003929"},{"key":"e_1_2_10_10_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2016.1188095"},{"key":"e_1_2_10_11_2","doi-asserted-by":"publisher","DOI":"10.1080\/14786419.2021.2021201"},{"key":"e_1_2_10_12_2","doi-asserted-by":"publisher","DOI":"10.5897\/jmpr11.1424"},{"key":"e_1_2_10_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fsi.2021.12.004"},{"key":"e_1_2_10_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0061922"},{"key":"e_1_2_10_15_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40816-021-00279-z"},{"key":"e_1_2_10_16_2","unstructured":"ZhangJ.-G. 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