{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:59:19Z","timestamp":1790607559247,"version":"4.1.0"},"reference-count":76,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T00:00:00Z","timestamp":1790553600000},"content-version":"vor","delay-in-days":270,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004242","name":"Princess Nourah Bint Abdulrahman University","doi-asserted-by":"publisher","award":["PNURSP2026R158"],"award-info":[{"award-number":["PNURSP2026R158"]}],"id":[{"id":"10.13039\/501100004242","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Embelia ribes<\/jats:italic>\n                    (ER) is well regarded in herbal medicine for its therapeutic properties, making the development of efficient methods to obtain its bioactive extracts of high yield essential. This study evaluated and optimized three extraction techniques, microwave\u2010pretreated ultrasound\u2010assisted extraction (MP\u2010UAE), ultrasound\u2010assisted extraction (UAE), and microwave\u2010assisted extraction (MAE), to isolate bioactive compounds from ER seeds. Embelin, a primary bioactive, was quantified in the optimized extracts, while 13 bioactives, including embelin, were analyzed through in silico studies targeting HCV NS3 protease. Process optimization using response surface methodology (RSM) focused on key parameters: total phenolic content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity (DRSA), and ABTS radical scavenging activity (ARSA). Experimental results validated the models, with MP\u2010UAE yielding the highest TPC (183.00\u2009mg GAE\/g DW), TFC (54.54\u2009mg RE\/g DW), DRSA (72.80%), and ARSA (81.33%). Optimized conditions included microwave power of 238\u2009W, 48\u2010min sonication, and a 19\u2010mL\/g solvent\u2010to\u2010plant ratio for MP\u2010UAE. Embelin content, measured spectrophotometrically, was highest in MP\u2010UAE at 39.98\u2009\u00b1\u20090.97\u2009mg\/g DW. Molecular docking using Maestro 12.5 identified amentoflavone (\u22128.991\u2009kcal\/mol), embelin (\u22128.152\u2009kcal\/mol), quercetin (\u22128.107\u2009kcal\/mol), and aloe\u2010emodin (\u22128.014\u2009kcal\/mol) as top compounds, surpassing the standard drug boceprevir (\u22125.342\u2009kcal\/mol). This study establishes a green, efficient extraction method for ER seed bioactives, highlighting the antiviral potential of embelin and contributing to herb\u2010based pharmaceutical advancements, particularly in antiviral drug development.\n                  <\/jats:p>","DOI":"10.1155\/jamc\/5570481","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T14:33:48Z","timestamp":1790606028000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Synergistic Polyphenol Recovery From\n                    <i>Embelia ribes<\/i>\n                    Using MP\u2010UAE, UAE, and MAE and In Silico Studies Against HCV Protease"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4312-3089","authenticated-orcid":false,"given":"Faiza","family":"Imtiaz","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3518-7181","authenticated-orcid":false,"given":"Dildar","family":"Ahmed","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saira","family":"Zumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3160-6008","authenticated-orcid":false,"given":"Samiah H.","family":"Al-Mijalli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7393-8065","authenticated-orcid":false,"given":"Munawar","family":"Iqbal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,28]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.5530\/pj.2012.27.2"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.31033\/ijrasb.8.2.31"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.58803\/fahn.v1i1.9"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/chromsci\/bmz097"},{"key":"e_1_2_11_5_2","doi-asserted-by":"crossref","first-page":"23","DOI":"10.9790\/3008-09332330","article-title":"Ethnopharmacological Uses of Embelia ribes Burm. 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