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In Kenya, data on the prevalence and genetic diversity of canine gastrointestinal helminths are scarce. This study investigated the prevalence of zoonotic helminths in dogs and characterised the genetic diversity of canine hookworms in Machakos County, Kenya.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A cross\u2010sectional household\u2010based survey was conducted across three sublocations representing low, medium and high human population density. Faecal samples were collected from 104 dog\u2010owning households and examined using Mini\u2010FLOTAC and zinc chloride sieving\u2013flotation techniques. Hookworm species were identified using nested polymerase chain reaction targeting the mitochondrial cytochrome c oxidase subunit I gene, followed by DNA sequencing. Phylogenetic diversity of\n                      <jats:italic>Ancylostoma caninum<\/jats:italic>\n                      was assessed using median\u2010joining haplotype network analyses.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mini\u2010FLOTAC detected a higher prevalence of hookworm (89.4% vs. 81.6%),\n                      <jats:italic>Toxocara<\/jats:italic>\n                      spp. (45.2% vs. 19.7%) and taeniids (29.8% vs. 5.3%) compared to zinc chloride sieving\u2013flotation. The latter identified additional parasites, including\n                      <jats:italic>Trichuris<\/jats:italic>\n                      spp. (9.2%),\n                      <jats:italic>Spirocerca lupi<\/jats:italic>\n                      (3.9%),\n                      <jats:italic>Alaria<\/jats:italic>\n                      spp. (1.3%) and\n                      <jats:italic>Clonorchis sinensis<\/jats:italic>\n                      (2.6%).\n                      <jats:italic>A. caninum<\/jats:italic>\n                      was the sole hookworm species identified, alongside genetically distinct\n                      <jats:italic>A. caninum<\/jats:italic>\n                      \u2010like lineages. High genetic variability was observed, comprising 80 polymorphic sites and 40 haplotypes, of which 31 were singletons, seven occurred in two sequences each and two were represented by five and six sequences. Haplotype network and phylogenetic analyses revealed high genetic diversity and weak population structuring in\n                      <jats:italic>A. caninum<\/jats:italic>\n                      , with Kenyan isolates clustering with global lineages while exhibiting local diversification. We also report the first molecular confirmation of\n                      <jats:italic>Angiostrongylus vasorum<\/jats:italic>\n                      in domestic dogs in Kenya.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These findings underscore dogs as key reservoirs of zoonotic helminths and highlight the need for strengthened surveillance and integrated One Health strategies incorporating molecular diagnostics and targeted education within dog health programmes in line with the WHO NTD Roadmap.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/japr\/1863975","type":"journal-article","created":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T09:39:06Z","timestamp":1790156346000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Prevalence of Zoonotic Parasites and Genetic Diversity of\n                    <i>Ancylostoma caninum<\/i>\n                    in Dogs From Machakos County, Kenya"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6873-7736","authenticated-orcid":false,"given":"Camille","family":"Glazer","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-7828-6534","authenticated-orcid":false,"given":"Nicholas","family":"Bor","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2402-7913","authenticated-orcid":false,"given":"Erastus","family":"Mulinge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6467-4352","authenticated-orcid":false,"given":"Maurice 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