{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T08:06:14Z","timestamp":1779177974148,"version":"3.51.4"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":138,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Parasitology Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"\n Background<\/jats:title>\n \n Toxoplasma gondii<\/jats:italic>\n is a globally prevalent protozoan parasite associated with adverse pregnancy outcomes, including miscarriage and congenital abnormalities. This study investigated the molecular presence of\n T. gondii<\/jats:italic>\n in placental tissues from women with spontaneous abortion and explored potential associated risk factors in Birjand, Eastern Iran.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n A total of 100 placental or fetal tissue samples were collected from women with confirmed spontaneous abortion during 2022\u20132023. Genomic DNA was extracted and analyzed using nested PCR targeting the B1 gene of\n T. gondii<\/jats:italic>\n . Demographic, obstetric, and behavioral data were also collected and statistically analyzed.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n T. gondii<\/jats:italic>\n DNA was detected in 8% of samples. A significant association was observed between infection and contact with domestic animals, including pet care (\n p<\/jats:italic>\n < 0.05). No significant relationships were identified with maternal age, place of residence, or prior abortion history (\n p<\/jats:italic>\n > 0.05). Notably, all positive cases were identified in pregnancies beyond 8 weeks of gestation (\n p<\/jats:italic>\n < 0.05), suggesting an increased detectability or susceptibility in later gestational stages.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n These findings indicate that\n T. gondii<\/jats:italic>\n infection may contribute to a subset of spontaneous abortions in this region. The results highlight the importance of targeted preventive strategies, including improved hygiene practices and awareness regarding animal exposure. Further large\u2010scale studies integrating both molecular and serological approaches are warranted to better elucidate the role of\n T. gondii<\/jats:italic>\n in adverse pregnancy outcomes.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1155\/japr\/4639653","type":"journal-article","created":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T07:11:10Z","timestamp":1779174670000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Molecular Identification of\n Toxoplasma gondii<\/i>\n Isolates From Spontaneous Abortion Placentas in Women in Eastern Iran"],"prefix":"10.1155","volume":"2026","author":[{"given":"Peyman","family":"Bagherzadeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3298-0432","authenticated-orcid":false,"given":"Seyed Mohammad","family":"Mousavi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8216-0734","authenticated-orcid":false,"given":"Amir","family":"Tavakoli Kareshk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahmoodreza","family":"Behravan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0754-9998","authenticated-orcid":false,"given":"Davod","family":"Javanmard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2002.00485.x"},{"key":"e_1_2_12_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16412-X"},{"key":"e_1_2_12_3_2","first-page":"382","article-title":"The First Survey of Isolation and Molecular Typing of Toxoplasma gondii by Bioassay and PCR Method in BALB\/c Mice in Camels (Camelus dromedarius) From Eastern Iran","volume":"13","author":"Tavakoli Kareshk A.","year":"2018","journal-title":"Iranian Journal of Parasitology"},{"key":"e_1_2_12_4_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.00389"},{"key":"e_1_2_12_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(01)00125-4"},{"key":"e_1_2_12_6_2","first-page":"1099","volume-title":"Handbook of Clinical Neurology","author":"Kieffer F.","year":"2013"},{"key":"e_1_2_12_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9378(71)90892-1"},{"key":"e_1_2_12_8_2","doi-asserted-by":"publisher","DOI":"10.1080\/01443610220130616"},{"key":"e_1_2_12_9_2","doi-asserted-by":"publisher","DOI":"10.5812\/modernc-127872"},{"key":"e_1_2_12_10_2","doi-asserted-by":"crossref","DOI":"10.1201\/9781420092370","volume-title":"Toxoplasmosis of Animals and Humans","author":"Dubey J. 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