{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T12:10:01Z","timestamp":1722341401578},"reference-count":0,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:p>Type I signal peptidases are important membrane\u2010bound serine\nproteases responsible for the cleavage of the signal peptide of\nthe proteins. These enzymes are unique serine proteases that\ncarry out catalysis using a serine\/lysine catalytic dyad. In the\npresent study, we report the isolation of type I signal peptidase\nfrom the malaria parasites <jats:italic>Plasmodium falciparum<\/jats:italic>,\n<jats:italic>Plasmodium knowlesi<\/jats:italic>, and <jats:italic>Plasmodium yoelii<\/jats:italic> and\nsome characterization of type I signal peptidase of\n<jats:italic>Plasmodium falciparum<\/jats:italic>. We show that these enzymes are\nhomologous to signal peptidases from various sources and also\ncontain the conserved boxes present in other type I signal\npeptidases. The type I signal peptidase from <jats:italic>P falciparum<\/jats:italic>\nis an intron\u2010less and a single\u2010copy gene. The results also show\nthat the enzyme from <jats:italic>Plasmodium falciparum<\/jats:italic> is subject to\nself\u2010cleavage and it has been demonstrated to possess type I\nsignal peptidase activity in <jats:italic>E coli<\/jats:italic> preprotein processing\nin vivo by complementation assay. This study will be helpful in\nunderstanding one of the important metabolic pathways \u201cthe\nsecretory pathway\u201d in the parasite and should make an important\ncontribution in understanding the complex process of protein\ntargeting in the parasite.<\/jats:p>","DOI":"10.1155\/jbb.2005.301","type":"journal-article","created":{"date-parts":[[2005,12,21]],"date-time":"2005-12-21T12:55:00Z","timestamp":1135169700000},"page":"301-309","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Isolation and Characterization of Type I Signal Peptidase ofDifferent Malaria Parasites"],"prefix":"10.1155","volume":"2005","author":[{"given":"Sutikshan","family":"Sharma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arun","family":"Pradhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virander S.","family":"Chauhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renu","family":"Tuteja","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,12,14]]},"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2005\/808623.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2005\/808623.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/JBB.2005.301","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T11:51:44Z","timestamp":1722340304000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/JBB.2005.301"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1155\/JBB.2005.301"],"URL":"https:\/\/doi.org\/10.1155\/jbb.2005.301","archive":["Portico"],"relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"type":"print","value":"2314-6133"},{"type":"electronic","value":"2314-6141"}],"subject":[],"published":{"date-parts":[[2005,1]]},"assertion":[{"value":"2005-02-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-07-14","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2005-12-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}