{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T15:29:39Z","timestamp":1781018979607,"version":"3.54.1"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T00:00:00Z","timestamp":1775865600000},"content-version":"vor","delay-in-days":100,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      CD14\n                      <jats:sup>+<\/jats:sup>\n                      CD16\n                      <jats:sup>++<\/jats:sup>\n                      6\u2010sulfo LacNAc (Slan)\n                      <jats:sup>+<\/jats:sup>\n                      monocytes represent a specialized nonclassical subset with reported pro\u2010inflammatory and regulatory features. However, their capacity to modulate classical monocytes and T lymphocytes under apoptotic and inflammatory conditions remains incompletely defined.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We employed a coculture reconstitution model using flow cytometry\u2014sorted CD14\n                      <jats:sup>+<\/jats:sup>\n                      CD16\n                      <jats:sup>++<\/jats:sup>\n                      Slan\n                      <jats:sup>+<\/jats:sup>\n                      monocytes and CD14\n                      <jats:sup>++<\/jats:sup>\n                      CD16\n                      <jats:sup>-<\/jats:sup>\n                      Slan\n                      <jats:sup>\u2212<\/jats:sup>\n                      classical monocytes at defined ratios. Cultures were stimulated with lipopolysaccharide (LPS) or K562\u2010derived apoptotic bodies (ABs). Monocyte surface marker expression was evaluated by flow cytometry, and cytokine concentrations were quantified using multiplex bead\u2010based assays. The impact of Slan\n                      <jats:sup>+<\/jats:sup>\n                      monocytes on CD3\n                      <jats:sup>+<\/jats:sup>\n                      T cell proliferation and cytokine production was assessed following monocyte\u2013T cell coculture.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      CD14\n                      <jats:sup>+<\/jats:sup>\n                      CD16\n                      <jats:sup>++<\/jats:sup>\n                      Slan\n                      <jats:sup>+<\/jats:sup>\n                      monocytes exhibited enhanced uptake of ABs and distinct phenotypic modulation upon stimulation. Increasing proportions of Slan\n                      <jats:sup>+<\/jats:sup>\n                      cells induced higher HLA\u2010DR and CCR5 expression on classical monocytes. LPS stimulation promoted TNF, IL\u201012, IL\u20106, and CCL2 production, whereas AB exposure favored IL\u201010 and IL\u201017 accumulation. In coculture assays, Slan\n                      <jats:sup>+<\/jats:sup>\n                      monocytes reduced CD4\n                      <jats:sup>+<\/jats:sup>\n                      and CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cell proliferation and altered T cell cytokine profiles in a stimulus\u2010dependent manner.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      CD14\n                      <jats:sup>+<\/jats:sup>\n                      CD16\n                      <jats:sup>++<\/jats:sup>\n                      Slan\n                      <jats:sup>+<\/jats:sup>\n                      monocytes exert a dose\u2010 and context\u2010dependent modulatory effect on classical monocytes and T lymphocytes in vitro, displaying both inflammatory and regulatory properties depending on the stimulus. These findings highlight their functional plasticity within the human monocyte compartment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/jimr\/3322498","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T14:35:43Z","timestamp":1775918143000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Regulatory Effects of CD14\n                    <sup>+<\/sup>\n                    CD16\n                    <sup>++<\/sup>\n                    Slan\n                    <sup>+<\/sup>\n                    Monocytes on Classical Monocytes and T Lymphocytes in Response to Inflammatory and Phagocytic Stimuli"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9176-939X","authenticated-orcid":false,"given":"Cristian A.","family":"Anacona","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1968-4647","authenticated-orcid":false,"given":"Karen","family":"\u00c1lvarez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0647-0906","authenticated-orcid":false,"given":"Gloria","family":"V\u00e1squez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7791-9328","authenticated-orcid":false,"given":"Mauricio","family":"Rojas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,11]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V74.7.2527.2527"},{"key":"e_1_2_13_2_2","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1002\/1521-4141(200101)31:1<48::AID-IMMU48>3.0.CO;2-5","article-title":"Identification of a Novel Dendritic Cell-Like Subset of CD64(+) \/ CD16(+) Blood Monocytes","volume":"31","author":"Grage-Griebenow E.","year":"2001","journal-title":"European Journal of Immunology"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-02-258558"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-01-326827"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1287656"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2015-06-651331"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.02052"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.96492"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4029"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-17-2344"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.38860"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2019.03.005"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-2791"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2010.09.003"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2020.577971"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/cei.13259"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-017-0024-8"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/s40348-018-0083-4"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2506"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.03.020"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1080\/17435889.2024.2415877"},{"key":"e_1_2_13_22_2","doi-asserted-by":"publisher","DOI":"10.3892\/etm.2020.8408"},{"key":"e_1_2_13_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.wneu.2022.03.025"},{"key":"e_1_2_13_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/cc8959"},{"key":"e_1_2_13_25_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0182427"},{"key":"e_1_2_13_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.163.3.1435"},{"key":"e_1_2_13_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.42.6.704"},{"key":"e_1_2_13_28_2","doi-asserted-by":"publisher","DOI":"10.1203\/01.PDR.0000059749.82140.4A"},{"key":"e_1_2_13_29_2","doi-asserted-by":"publisher","DOI":"10.2337\/db11-1506"},{"key":"e_1_2_13_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2016.116"},{"key":"e_1_2_13_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-022-01289-w"},{"key":"e_1_2_13_32_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.1.189"},{"key":"e_1_2_13_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2010.12.009"},{"key":"e_1_2_13_34_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0100734"},{"key":"e_1_2_13_35_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1998.00481.x"},{"key":"e_1_2_13_36_2","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2018.1444411"},{"key":"e_1_2_13_37_2","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2015.1008342"},{"key":"e_1_2_13_38_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.2001194"},{"key":"e_1_2_13_39_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0176460"},{"key":"e_1_2_13_40_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0122529"},{"key":"e_1_2_13_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00226"},{"key":"e_1_2_13_42_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2567.2010.03336.x"},{"key":"e_1_2_13_43_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01352"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jimr\/3322498","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/jimr\/3322498","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jimr\/3322498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T14:35:44Z","timestamp":1775918144000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/jimr\/3322498"}},"subtitle":[],"editor":[{"given":"Poorani","family":"Gurumallesh Prabu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/jimr\/3322498"],"URL":"https:\/\/doi.org\/10.1155\/jimr\/3322498","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"3322498"}}