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<jats:sup>+<\/jats:sup>\n                    T cells. Dendritic cell (DC)\u2010based immunotherapeutic approaches have emerged as potential strategies to enhance antigen\u2010specific immune responses against HIV\u20101. In this context, alpha\u2010type\u20101 polarized DCs (\u03b1DC1) are notable for their capacity to produce interleukin\u201012p70 (IL\u201012p70) and induce robust Th1 and cytotoxic T lymphocyte (CTL) responses. However, clinical outcomes have been inconsistent, often influenced by host factors and methodological variability in DC generation and antigen delivery. The high genetic diversity of HIV poses challenges for vaccine development, whereas conserved regions such as gag and pol represent promising immunogenic targets. The present study aimed to evaluate a set of previously identified conserved HIV\u20101 peptides for their ability to stimulate HIV\u2010specific T cell responses when presented by \u03b1DC1 in vitro in coculture assays using samples from a Brazilian cohort, as part of a preparatory phase for a clinical trial. For this purpose, monocytes were differentiated into \u03b1DC1 using IL\u20104 and GM\u2010CSF. On Day 5, the cells were pulsed with 14\u201321mer HIV\u20101 peptide pools (gag and pol) and matured by the addition of IFN\u2010\u03b1, IFN\u2010\u03b3, IL\u20101\u03b2, and TNF\u2010\u03b1. After 48\u2009h, \u03b1DC1 were harvested and cocultured with autologous T cells for 6 or 20 days. Pulsed\u2010\u03b1DC1 were able to induce immune responses through the production of IFN\u2010\u03b3 in T cells, with the subpopulation of effector memory CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells (\n                    <jats:italic>T<\/jats:italic>\n                    <jats:sub>EM<\/jats:sub>\n                    ) being responsible for the production of this cytokine after reexposure to the HIV antigens.\n                  <\/jats:p>","DOI":"10.1155\/jimr\/5457670","type":"journal-article","created":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T12:29:13Z","timestamp":1778848153000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Induction of HIV\u2010Specific T Cell Responses Using \u03b1DC1 Pulsed With Conserved HIV\u20101 Peptides"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8542-802X","authenticated-orcid":false,"given":"La\u00eds Teodoro","family":"Da Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3258-8315","authenticated-orcid":false,"given":"Marina Mazzilli","family":"Ortega","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-9789-7861","authenticated-orcid":false,"given":"Silvia","family":"de Jesus Mota","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5474-254X","authenticated-orcid":false,"given":"Guilherme","family":"Castellani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2567-5808","authenticated-orcid":false,"given":"Dennyson Leandro M.","family":"Fonseca","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6469-3123","authenticated-orcid":false,"given":"Larissa Danielle Bahls","family":"Pinto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7069-1017","authenticated-orcid":false,"given":"Gabriela Justamante Handel","family":"Schmitz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3099-2986","authenticated-orcid":false,"given":"Isabela","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7863-2896","authenticated-orcid":false,"given":"Theo","family":"Leite","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6015-9924","authenticated-orcid":false,"given":"Mariana Amelia","family":"Monteiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0118-5361","authenticated-orcid":false,"given":"Sandra Marcia","family":"Muxel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5501-503X","authenticated-orcid":false,"given":"Robbie B.","family":"Mailliard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8707-4904","authenticated-orcid":false,"given":"Alberto Jos\u00e9","family":"da Silva Duarte","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8791-9233","authenticated-orcid":false,"given":"Telma Miyuki","family":"Oshiro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,15]]},"reference":[{"key":"e_1_2_11_1_2","volume-title":"Highly Active Antiretroviral Therapy (HAART)","author":"Eggleton J. 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