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Here, it was found that pretreatment of macrophages with GT can significantly attenuate the conversion of LC3\u2010II. In parallel, the expression of Rubicon, the putative indicator of LAP in macrophages, was dampened in a similar trend. Loss of ability to produce GT made the conidia of\n                    <jats:italic>gliP<\/jats:italic>\n                    \u0394 mutant of\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    induce more LC3\u2010II conversion in THP1 cells, which could be inhibited by exogenous GT. Comparative transcriptomic analysis showed that GT can promote the expression of MAPK10, and the calcium\u2010release regulatory pathway was enriched between the differentially expressed genes. Further, GT promotes the release of ROS at a concentration of 50\u2009ng\/mL, and inhibition by GT on LC3\u2010II production in macrophages during\n                    <jats:italic>A. fumigatus<\/jats:italic>\n                    infection could be restored by pretreatment with calcium inhibitors but not affected by the inhibitors for JNK and siRNA for MAKP10. Collectively, our data demonstrated that GT exerts an inhibitory effect on LC3\u2010associated phagocytosis in macrophages via a calcium\u2010dependent mechanism, and MAPK10 exists downstream of LAP.\n                  <\/jats:p>","DOI":"10.1155\/jimr\/5542735","type":"journal-article","created":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T04:49:57Z","timestamp":1777524597000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["<i>Aspergillus fumigatus<\/i>\n                    Gliotoxin Inhibits LC3\u2010Associated Phagocytosis in Macrophages in a Calcium\u2010Dependent Manner"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-4699-7461","authenticated-orcid":false,"given":"Yuqing","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9545-1952","authenticated-orcid":false,"given":"Fangyan","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1233-3892","authenticated-orcid":false,"given":"Ling","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3138-6722","authenticated-orcid":false,"given":"Caopei","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2822-2488","authenticated-orcid":false,"given":"Yu","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1214-594X","authenticated-orcid":false,"given":"Yulin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9013-8414","authenticated-orcid":false,"given":"Li","family":"Han","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,29]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1111\/imj.15591","article-title":"Consensus Guidelines for the Diagnosis and Management of Invasive Aspergillosis, 2021","volume":"51","author":"Douglas A. 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