{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T07:57:37Z","timestamp":1790409457555,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":175,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274542"],"award-info":[{"award-number":["82274542"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82274615"],"award-info":[{"award-number":["82274615"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82205137"],"award-info":[{"award-number":["82205137"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009659","name":"Guangzhou Medical University","doi-asserted-by":"publisher","award":["2023RC10"],"award-info":[{"award-number":["2023RC10"]}],"id":[{"id":"10.13039\/100009659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010618","name":"Guangzhou University of Chinese Medicine","doi-asserted-by":"publisher","award":["A3-0317-24-429-006"],"award-info":[{"award-number":["A3-0317-24-429-006"]}],"id":[{"id":"10.13039\/501100010618","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Intervertebral disc degeneration (IVDD) is a major cause of chronic low\u2010back pain (LBP). The inflammatory environment and senescence of nucleus pulposus cells (NPCs) within the intervertebral disc (IVD) are critical processes in IVDD. Plastrum testudinis extract (PTE) has been proven to have anti\u2010inflammatory qualities; however it is unknown whether it can impact the progression of IVDD and NPC senescence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We investigated the effects of PTE on NPC senescence induced by tert\u2010butyl hydrogen peroxide (TBHP) in vitro. Cell viability, senescence\u2010associated secretory phenotype (SASP), inflammatory factors, and extracellular matrix (ECM) were assessed. The NF\u2010\u03baB pathway was activated using lipopolysaccharide (LPS) to explore underlying mechanisms. In vivo studies used a senile mouse with IVDD, evaluating disc structure and inflammatory factors after PTE treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>TBHP stimulation rapidly induced NPC senescence, increased inflammatory factors, and disrupted ECM anabolic balance; these effects were partially alleviated by PTE treatment. PTE inhibited NF\u2010\u03baB pathway activation and prevented p65 nuclear translocation. In senile mice, PTE treatment partially preserved IVD structural integrity. Immunohistochemistry showed PTE inhibited IL\u20101\u03b2 expression, while immunofluorescence demonstrated increased aggrecan expression following PTE treatment.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PTE delays IVDD progression through anti\u2010inflammatory effects, specifically by inhibiting NF\u2010\u03baB pathway activation and reducing inflammatory factor expression. These findings suggest PTE could be a promising therapeutic agent for IVDD treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/jimr\/6396887","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T13:58:20Z","timestamp":1782395900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Plastrum Testudinis Extract Ameliorates Intervertebral Disc Degeneration by Suppressing NF\u2010\u03baB Mediated Senescence and Inflammation"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7123-5726","authenticated-orcid":false,"given":"Jiahui","family":"He","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-6379-9366","authenticated-orcid":false,"given":"Yanchi","family":"Gan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1777-3790","authenticated-orcid":false,"given":"Peng","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-4860-3837","authenticated-orcid":false,"given":"Yan","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3246-0007","authenticated-orcid":false,"given":"Zhaojun","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9135-2666","authenticated-orcid":false,"given":"Qi","family":"Shang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7300-5345","authenticated-orcid":false,"given":"Honglin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7603-1645","authenticated-orcid":false,"given":"Guifeng","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3214-4634","authenticated-orcid":false,"given":"Wenhua","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8940-7912","authenticated-orcid":false,"given":"Gengyang","family":"Shen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6004-920X","authenticated-orcid":false,"given":"Hui","family":"Ren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8009-0282","authenticated-orcid":false,"given":"Xiaobing","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1374-2016","authenticated-orcid":false,"given":"Henian","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0263204"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2021.07.012"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijerph19063721"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsp2.1076"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41584-020-00568-w"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202101483"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2019.100978"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/s12276-022-00753-9"},{"key":"e_1_2_14_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/adhm.202100596"},{"key":"e_1_2_14_10_2","doi-asserted-by":"publisher","DOI":"10.22203\/eCM.v041a46"},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13057"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-021-03906-7"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.111804"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.923386"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202002_20387"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114195"},{"key":"e_1_2_14_17_2","doi-asserted-by":"publisher","DOI":"10.1159\/000491541"},{"key":"e_1_2_14_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-25453-2"},{"key":"e_1_2_14_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/6815620"},{"key":"e_1_2_14_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1747-0285.2012.01361.x"},{"key":"e_1_2_14_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2025.1549834"},{"key":"e_1_2_14_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113946"},{"key":"e_1_2_14_23_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.07-9574LSF"},{"key":"e_1_2_14_24_2","doi-asserted-by":"publisher","DOI":"10.4103\/0976-0105.177703"},{"key":"e_1_2_14_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2020.09.003"},{"key":"e_1_2_14_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep21146"},{"key":"e_1_2_14_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.5"},{"key":"e_1_2_14_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-024-00373-1"},{"key":"e_1_2_14_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-32552-1"},{"key":"e_1_2_14_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13323"},{"key":"e_1_2_14_31_2","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.52570"},{"key":"e_1_2_14_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.114711"},{"key":"e_1_2_14_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2024.107119"},{"key":"e_1_2_14_34_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13018-025-06075-6"},{"key":"e_1_2_14_35_2","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.13448"},{"key":"e_1_2_14_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsp2.70089"},{"key":"e_1_2_14_37_2","doi-asserted-by":"publisher","DOI":"10.1155\/sci\/3373211"},{"key":"e_1_2_14_38_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.787278"},{"key":"e_1_2_14_39_2","doi-asserted-by":"publisher","DOI":"10.1002\/jsp2.70058"},{"key":"e_1_2_14_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2025.01.013"},{"key":"e_1_2_14_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arr.2020.101227"},{"key":"e_1_2_14_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jot.2024.02.003"},{"key":"e_1_2_14_43_2","doi-asserted-by":"publisher","DOI":"10.1155\/2022\/8672969"},{"key":"e_1_2_14_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bioactmat.2021.12.007"},{"key":"e_1_2_14_45_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-025-02429-8"},{"key":"e_1_2_14_46_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2022.942435"},{"key":"e_1_2_14_47_2","doi-asserted-by":"publisher","DOI":"10.3390\/ph16101482"},{"key":"e_1_2_14_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111832"},{"key":"e_1_2_14_49_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41413-020-00125-x"},{"key":"e_1_2_14_50_2","doi-asserted-by":"publisher","DOI":"10.1134\/S1819712421020045"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jimr\/6396887","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/jimr\/6396887","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jimr\/6396887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T13:58:22Z","timestamp":1782395902000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/jimr\/6396887"}},"subtitle":[],"editor":[{"given":"Poorani","family":"Gurumallesh Prabu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":50,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/jimr\/6396887"],"URL":"https:\/\/doi.org\/10.1155\/jimr\/6396887","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2026-01-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-25","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6396887"}}