{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T13:10:51Z","timestamp":1789996251956,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":263,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005047","name":"Natural Science Foundation of Liaoning Province","doi-asserted-by":"publisher","award":["2024-MS-201"],"award-info":[{"award-number":["2024-MS-201"]}],"id":[{"id":"10.13039\/501100005047","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>Mastitis is a prevalent inflammatory disorder in women, this condition exhibits a particularly elevated prevalence among breastfeeding women, which significantly compromises mammary health and lactation function. This research explored the anti\u2010inflammatory properties of YWJ\u20106 in lipopolysaccharide (LPS)\u2010induced mastitis, its protective role on the blood\u2013milk barrier (BMB), and the underlying mechanisms. An LPS\u2010induced mastitis rat model was established. H&amp;E staining assessed mammary tissue damage, and myeloperoxidase (MPO) activity was measured. RT\u2010qPCR and IHC were used to demonstrate interleukin 1\u03b2 (IL\u20101\u03b2), interleukin 6 (IL\u20106), and tumor necrosis factor alpha (TNF\u03b1) expression. These data indicated that inflammatory injury was alleviated. Following drug treatment, MPO expression and the mRNA\/protein expression of these inflammatory factors were suppressed. Immunofluorescence staining analyzed the distribution of Zonula Occludens\u20101 (ZO\u20101), Occludin, and Claudin\u20103. WB quantitatively detected ZO\u20101 and Occludin expression, confirming that YWJ\u20106 promoted tight junction (TJ) protein expression restoration and distribution integrity, repairing the damaged BMB. Consistently, Evans blue extravasation assay demonstrated that YWJ\u20106 reduced mammary vascular permeability, providing direct functional evidence for BMB protection. mIHC detected the colocalization of CD68, MPO, and nuclear factor\u2010kappa B (NF\u2010\u03baB) p65 subunit (NF\u2010\u03baB p65), showing that YWJ\u20106 modulated inflammatory cell infiltration and p65 activation. RT\u2010qPCR, WB, and ELISA assays in HC11 and RAW264.7 cells confirmed that YWJ\u20106 downregulated inducible nitric oxide synthase (iNOS), cyclooxygenase\u20102 (COX\u20102) and IL\u20101\u03b2, IL\u20106 and TNF\u2010\u03b1 expression in HC11 cells, and suppressed agent responsible for inflammation secretion in RAW264.7 cells. WB and immunofluorescence showed that YWJ\u20106 inhibited inhibitor of NF\u2010\u03baB alpha (I\u03baB\u03b1) and NF\u2010\u03baB p65 phosphorylation in both HC11 and RAW264.7 cells. In conclusion, YWJ\u20106 exerts dual protective effects by targeting NF\u2010\u03baB signaling pathway activation, providing experimental evidence for mastitis treatment in future.<\/jats:p>","DOI":"10.1155\/jimr\/7518397","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:24:37Z","timestamp":1789993477000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Therapeutic Potential of Thiosemicarbazone Derivative in LPS\u2010Induced Mastitis: Dual Modulation of Inflammatory Response and Blood\u2013Milk Barrier Integrity"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-5197-6651","authenticated-orcid":false,"given":"Ling","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3241-5402","authenticated-orcid":false,"given":"Yining","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7311-1260","authenticated-orcid":false,"given":"Jia","family":"Liang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-7984-6490","authenticated-orcid":false,"given":"Ying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7188-1496","authenticated-orcid":false,"given":"Wei","family":"Tao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"crossref","DOI":"10.3389\/fmicb.2017.01258","article-title":"Identification of Emerging Human Mastitis Pathogens by MALDI-TOF and Assessment of Their Antibiotic Resistance Patterns","volume":"8","author":"Mar\u00edn M.","year":"2017","journal-title":"Original Research. 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