{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T00:37:45Z","timestamp":1773794265401,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T00:00:00Z","timestamp":1764201600000},"content-version":"vor","delay-in-days":330,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002879","name":"Canadian HIV Trials Network, Canadian Institutes of Health Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002879","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008572","name":"University of Ottawa","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008572","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Infection with HIV alters \u03b3\u03b4 T cells, and while these changes have been well documented in blood, they are less well understood in the gut.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Phenotype (specifically HIV coreceptor expression) and polyfunctionality, as defined by concomitant cytokine expression, were evaluated in \u03b3\u03b4 T cells in the blood and gut of effectively treated people living with HIV (PLWH) and people without HIV (PWOH). Mononuclear cells were isolated from blood and recto\u2010sigmoid colon biopsy tissue, and \u03b3\u03b4 T cells were evaluated by flow cytometry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The expression of \u03b14\u03b27 was significantly higher in gut\u2010derived \u03b3\u03b4 T cells of PLWH compared to PWOH and blood\u2010derived cells of both groups. The polyfunctionality profile of gut\u2010derived \u03b3\u03b4 T cells in PLWH was also different than that of PWOH, with significantly higher expression of IFN\u2010\u03b3 in the gut\u2010derived cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The alterations observed in gut\u2010derived \u03b3\u03b4 T cells from effectively treated PLWH suggest cellular function is not restored with prolonged antiretroviral therapy (ART) and may contribute to a chronic inflammatory state.<\/jats:p>\n                    <jats:p>\n                      <jats:bold>Trial Registration:<\/jats:bold>\n                      HAVARTI trial registration (ClinicalTrials.gov): NCT03147859\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/jimr\/9883892","type":"journal-article","created":{"date-parts":[[2025,11,27]],"date-time":"2025-11-27T09:50:22Z","timestamp":1764237022000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Failure of \u03b3\u03b4 T Cell Recovery in the Gut With Effective Anti\u2010HIV Therapy"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6173-0416","authenticated-orcid":false,"given":"Priscila O.","family":"Barros","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie C.","family":"Burke Schinkel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ameeta Lubina","family":"Nayak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittany","family":"Haas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tamara K.","family":"Berthoud","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michaeline","family":"McGuinty","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. William","family":"Cameron","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3102-4462","authenticated-orcid":false,"given":"Jonathan B.","family":"Angel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,27]]},"reference":[{"key":"e_1_2_16_1_2","doi-asserted-by":"publisher","DOI":"10.3390\/cells9040800"},{"key":"e_1_2_16_2_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601146"},{"key":"e_1_2_16_3_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-01-198028"},{"key":"e_1_2_16_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01275-07"},{"key":"e_1_2_16_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0129771"},{"key":"e_1_2_16_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0106064"},{"key":"e_1_2_16_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-34576-4"},{"key":"e_1_2_16_8_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.157.1.449"},{"key":"e_1_2_16_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/aji.12458"},{"key":"e_1_2_16_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF03401668"},{"key":"e_1_2_16_11_2","doi-asserted-by":"publisher","DOI":"10.1097\/QAD.0b013e3283244619"},{"key":"e_1_2_16_12_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2011-05-356535"},{"key":"e_1_2_16_13_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.881607"},{"key":"e_1_2_16_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005201"},{"key":"e_1_2_16_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.77.19.10456-10467.2003"},{"key":"e_1_2_16_16_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmjopen-2020-041359"},{"key":"e_1_2_16_17_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1336480"},{"key":"e_1_2_16_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cyto.a.21015"},{"key":"e_1_2_16_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41409-019-0462-z"},{"key":"e_1_2_16_20_2","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.ade3525"},{"key":"e_1_2_16_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/00126334-200106010-00002"},{"key":"e_1_2_16_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.161.6.3195"},{"key":"e_1_2_16_23_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-200202010-00003"},{"key":"e_1_2_16_24_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15646-0"},{"key":"e_1_2_16_25_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0911796106"},{"key":"e_1_2_16_26_2","doi-asserted-by":"publisher","DOI":"10.1111\/hiv.13254"},{"key":"e_1_2_16_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4690-9-S2-P271"},{"key":"e_1_2_16_28_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.647398"},{"key":"e_1_2_16_29_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-12-4818"},{"key":"e_1_2_16_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41385-018-0110-8"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jimr\/9883892","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/jimr\/9883892","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/jimr\/9883892","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T20:50:47Z","timestamp":1773780647000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/jimr\/9883892"}},"subtitle":[],"editor":[{"given":"Poorani","family":"Gurumallesh Prabu","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/jimr\/9883892"],"URL":"https:\/\/doi.org\/10.1155\/jimr\/9883892","archive":["Portico"],"relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"value":"2314-8861","type":"print"},{"value":"2314-7156","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2025-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-10-30","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-11-27","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"9883892"}}