{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T11:44:36Z","timestamp":1775821476465,"version":"3.50.1"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T00:00:00Z","timestamp":1775779200000},"content-version":"vor","delay-in-days":99,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Obesity"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>MicroRNA (miRNA) profiling of visceral adipose tissue in Type\u20102 diabetes mellitus (T2DM) remains limited. We compared the expression of obesity\u2010associated miRNAs in visceral fat from individuals with T2DM versus metabolically healthy obesity (MHO) and examined Raf kinase inhibitory protein (RKIP) as a candidate miR\u2010543 target.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Visceral fat biopsies were obtained from adults with T2DM (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20098) and MHO (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200911). Thirteen miRNAs previously linked to obesity were quantified, and RKIP expression was evaluated at the mRNA level and by immunohistochemistry, including phosphorylated RKIP (pRKIP).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Of the 13 miRNAs analyzed, miR\u201023a\u20103p and miR\u2010543 were upregulated in T2DM (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.032 and\n                      <jats:italic>p<\/jats:italic>\n                      = 0.009, respectively), whereas miR\u2010320a\u20103p was downregulated (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.009). RKIP mRNA levels did not differ between groups; however, in MHO adipocytes, RKIP mRNA correlated positively with miR\u2010543 expression (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.655,\n                      <jats:italic>p<\/jats:italic>\n                      = 0.034). Total RKIP protein was comparable between groups, while pRKIP was significantly higher in T2DM adipocytes (\n                      <jats:italic>p<\/jats:italic>\n                      = 0.012).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Posttranscriptional regulation in visceral adipocytes differs between T2DM and MHO. Increased miR\u2010543 and elevated pRKIP in T2DM suggest a rapid shift in regulatory signaling consistent with enhanced lipolysis and a proinflammatory milieu. Further studies are warranted to delineate the pRKIP\u2010associated pathways in adipose tissue remodeling in T2DM.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/jobe\/3230015","type":"journal-article","created":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T10:45:00Z","timestamp":1775817900000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Expression Analysis of Selected microRNAs in Diabetes Mellitus Visceral Fat 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