{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T06:45:21Z","timestamp":1783406721272,"version":"3.54.6"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T00:00:00Z","timestamp":1764633600000},"content-version":"vor","delay-in-days":335,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["21\/06669-6"],"award-info":[{"award-number":["21\/06669-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["22\/10392-2"],"award-info":[{"award-number":["22\/10392-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>COVID\u201019 continues to pose a major global health challenge. Despite its geographic distance from Brazil\u2019s major urban centers, Acre state has experienced notable outbreaks. This study assessed the detection and persistence of SARS\u2010CoV\u20102 in the rural settlement of Santa Luzia, located in the remote municipality of Cruzeiro do Sul, Acre state, Brazil.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In July 2022, a cross\u2010sectional survey was conducted at 40 sites from an ongoing environmental study, selected by deforestation patterns and proximity to health posts. Saliva samples were collected from residents aged 5\u201390\u2009years, followed by nucleic acid extraction and multiplex RT\u2010qPCR for SARS\u2010CoV\u20102 detection.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Among the 183 individuals from 40 participating families, an 8% positivity rate was observed, with variation by age and sex. In 30% of families, at least one member tested positive, indicating continued viral presence within the community. Spatial analysis using Global and Local Moran\u2019s I statistics showed a random distribution of positive samples, consistent with multiple introductions from nearby urban centers and intermittent local persistence.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These findings highlight the ongoing detection and persistence of SARS\u2010CoV\u20102 in a remote Amazonian community, underscoring the need for continued surveillance in geographically isolated populations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/jotm\/4872494","type":"journal-article","created":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T11:14:41Z","timestamp":1764674081000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["SARS\u2010CoV\u20102 Detection and Persistence in a Remote Amazonian Settlement"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3125-7717","authenticated-orcid":false,"given":"Glauco M.","family":"Silva","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8322-4960","authenticated-orcid":false,"given":"Roberto C.","family":"Ilacqua","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-1292-1196","authenticated-orcid":false,"given":"Franciely G.","family":"Gon\u00e7alves","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8150-3191","authenticated-orcid":false,"given":"Carla M.","family":"Santana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1792-4395","authenticated-orcid":false,"given":"Felipe T.","family":"Jord\u00e3o","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2809-0434","authenticated-orcid":false,"given":"Paula R.","family":"Prist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8579-5372","authenticated-orcid":false,"given":"Melissa S.","family":"Nolan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4436-432X","authenticated-orcid":false,"given":"Andreia F.","family":"Brilhante","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1178-3747","authenticated-orcid":false,"given":"Marcia A.","family":"Speran\u00e7a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7412-9390","authenticated-orcid":false,"given":"Gabriel Z.","family":"Laporta","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,12,2]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0820-9"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0822-7"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(20)30183-5"},{"key":"e_1_2_13_4_2","unstructured":"Johns Hopkins University COVID-19 Data Repository by the Center for Systems Science and Engineering https:\/\/github.com\/CSSEGISandData\/COVID-19."},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10709-004-4309-0"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1590\/s1678-9946202365020"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12936-019-2938-0"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4100-6"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0006070"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.36311\/jhgd.v31.12611"},{"key":"e_1_2_13_11_2","volume-title":"Spatial Evolution of the First Wave of COVID-19 in 2020 in the Municipality of Cruzeiro Do Sul, AC, Brazil (PhD Thesis)","author":"Silva G. 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