{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T14:01:46Z","timestamp":1777903306451,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T00:00:00Z","timestamp":1744588800000},"content-version":"vor","delay-in-days":103,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Currently, carbapenem\u2010resistant\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    (CR\u2010KP) strains, particularly those producing New Delhi metallo\u2010beta\u2010lactamase (NDM), are increasingly recognized as a significant threat to global health. The present study aimed to conduct a genomic analysis of an NDM\u20101\u2010producing CR\u2010KP strain isolated from patients with coronavirus disease of 2019 (COVID\u201019) admitted to the intensive care unit (ICU). The\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    isolate was obtained from the bronchoalveolar lavage fluid of a 68\u2009year\u2010old male patient hospitalized in the ICU with COVID\u201019 at Besat Hospital in Sanandaj, Iran. The minimum inhibitory concentrations (MICs) for 15 antibiotics were determined using the VITEK 2 system. Genomic analysis of the isolate was performed using whole genome sequencing. The CRKP\u201051 strain was identified as an extensively drug\u2010resistant (XDR) strain, exhibiting resistance to all tested antibiotics except tigecycline (MIC\u2009=\u20092\u2009\u03bcg\/mL). The highest resistance values were recorded against sulfamethoxazole\u2010trimethoprim (SXT), nitrofurantoin (NIT), and piperacillin\u2010tazobactam (TZP), with MICs of \u2265 320, 256\u2009\u03bcg\/mL, and \u2265 128\u2009\u03bcg\/mL, respectively. Multilocus sequence typing revealed that CRKP\u201051 belonged to sequence type 15 (ST15). The IncHI1B replicon type associated with this strain harbored several resistance genes, including\n                    <jats:italic>b<\/jats:italic>\n                    <jats:italic>l<\/jats:italic>\n                    <jats:italic>a<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>N<\/jats:italic>\n                      <jats:italic>D<\/jats:italic>\n                      <jats:italic>M<\/jats:italic>\n                      \u22121\n                    <\/jats:sub>\n                    <jats:italic>, armA<\/jats:italic>\n                    ,\n                    <jats:italic>msrE<\/jats:italic>\n                    ,\n                    <jats:italic>mphE<\/jats:italic>\n                    ,\n                    <jats:italic>BRP<\/jats:italic>\n                    (MBL),\n                    <jats:italic>b<\/jats:italic>\n                    <jats:italic>l<\/jats:italic>\n                    <jats:italic>a<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>O<\/jats:italic>\n                      <jats:italic>X<\/jats:italic>\n                      <jats:italic>A<\/jats:italic>\n                      \u22121\n                    <\/jats:sub>\n                    ,\n                    <jats:italic>aadA<\/jats:italic>\n                    2,\n                    <jats:italic>dfrA<\/jats:italic>\n                    12,\n                    <jats:italic>qnrB<\/jats:italic>\n                    1,\n                    <jats:italic>b<\/jats:italic>\n                    <jats:italic>l<\/jats:italic>\n                    <jats:italic>a<\/jats:italic>\n                    <jats:sub>\n                      <jats:italic>C<\/jats:italic>\n                      <jats:italic>T<\/jats:italic>\n                      <jats:italic>X<\/jats:italic>\n                      \u2212\n                      <jats:italic>M<\/jats:italic>\n                      \u221215\n                    <\/jats:sub>\n                    , and\n                    <jats:italic>cat<\/jats:italic>\n                    1. High\u2010risk\n                    <jats:italic>K. pneumoniae<\/jats:italic>\n                    clones, such as ST15, are increasingly associated with antimicrobial resistance and the emergence of XDR strains in ICUs. Additionally, the global dissemination of the NDM enzyme occurs through various plasmid replicon types. Therefore, monitoring local epidemiology is essential for the effectiveness of antimicrobial stewardship programs.\n                  <\/jats:p>","DOI":"10.1155\/jotm\/6616950","type":"journal-article","created":{"date-parts":[[2025,4,14]],"date-time":"2025-04-14T07:49:46Z","timestamp":1744616986000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genomic Characterization of NDM\u20101 Harboring Extensively\u2010Drug Resistance\n                    <i>Klebsiella pneumoniae<\/i>\n                    Isolate From ICU\u2010Admitted Patient With COVID\u201019"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8457-4057","authenticated-orcid":false,"given":"Abolfazl 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