{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:30:41Z","timestamp":1773927041880,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T00:00:00Z","timestamp":1767744000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100006261","name":"Taif University","doi-asserted-by":"publisher","award":["TU-DSPP-2024-26"],"award-info":[{"award-number":["TU-DSPP-2024-26"]}],"id":[{"id":"10.13039\/501100006261","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    <jats:italic>Ocimum basilicum<\/jats:italic>\n                    is an important alternative source to explore diverse anti\u2010infective compounds. In the present study, aqueous seed extract of\n                    <jats:italic>O. basilicum<\/jats:italic>\n                    was used to identify bioactive compounds with antihemolytic, thrombolytic, antivancomycin\u2010resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    and antivancomycin\u2010resistant\n                    <jats:italic>S. epidermidis<\/jats:italic>\n                    activity. Anti\u2010VRSA and anti\u2010VRSE activity of\n                    <jats:italic>O. basilicum<\/jats:italic>\n                    seed aqueous extract was evaluated by the well diffusion assay. Hemolytic and thrombolytic activities were performed using a 96\u2010well plate. Phytochemical identification was done by GC\u2010MS. ADMET and docking analyses with VanA ligase of VRSA and VRSE were also performed. The aqueous extract showed antibacterial activity against VRSA (12\u2009\u00b1\u20090.35\u2009mm) and VRSE (13\u2009\u00b1\u20090.11\u2009mm) isolates. The\n                    <jats:italic>O. basilicum<\/jats:italic>\n                    showed significantly less hemolysis (3.7\u2009\u00b1\u20090.24%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.00001) of red blood cells, reflecting low cytotoxicity as compared to the control (98\u2009\u00b1\u20090.44%). The\n                    <jats:italic>O. basilicum<\/jats:italic>\n                    seed extract exhibited significant thrombolytic activity (4.33\u2009\u00b1\u20090.2%,\n                    <jats:italic>p<\/jats:italic>\n                    \u2009&lt;\u20090.000429) as compared to the negative control (2\u2009\u00b1\u20090.34%). Among 23 identified compounds on GC\u2010MS, eight were reported for the first time in\n                    <jats:italic>O. basilicum<\/jats:italic>\n                    aqueous seed extract and processed for molecular docking. After favorable water solubility, pharmacokinetics, medicinal chemistry, and drug likeness, only two compounds, d\u2010glucopyranoside, 2,3,4,6\u2010di\u2010O\u2010(ethylboranediyl)\u20101\u2010O\u2010methyl and 4(3,4\u2010dihydroxy\u20102\u2010oxo\u2010butylamino) benzonitrile, were processed for molecular docking. The first one formed three hydrogen bonds with Leu\u2010259, Ser\u2010127, and His\u201049 residues of the VanA ligase. The second one formed two hydrogen bonds with Ser\u2010161 and Val\u2010160 residues of the VanA ligase. d\u2010Glucopyranoside, 2,3,4,6\u2010di\u2010O\u2010(ethylboranediyl)\u20101\u2010O\u2010methyl and 4(3,4\u2010dihydroxy\u20102\u2010oxo\u2010butylamino) benzonitrile. The\n                    <jats:italic>O. basilicum<\/jats:italic>\n                    seed extract has potential bioactivity, and the identified compounds are novel putative VanA ligase inhibitors. Further characterization of the bioactive compounds would help to explore therapeutic targets against VRSA and VRSE.\n                  <\/jats:p>","DOI":"10.1155\/jotm\/6640607","type":"journal-article","created":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T09:15:15Z","timestamp":1767777315000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antihemolytic and Thrombolytic Potential of\n                    <i>Ocimum basilicum<\/i>\n                    Seed Extract, Bioactive Compounds, and Docking With VanA Ligase in Vancomycin\u2010Resistant Staphylococci"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8156-2069","authenticated-orcid":false,"given":"Khalaf F.","family":"Alsharif","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6572-8428","authenticated-orcid":false,"given":"Hazir","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,1,7]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1234701"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2010.02.001"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6882-6-35"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/9780470123010.ch1"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/plants12244148"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.22038\/ijbms.2023.67466.14783"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.afres.2025.100859"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-023-04058-w"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27082377"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules29020388"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcab.2022.102556"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.thromres.2005.12.011"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.05.043"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiph.2021.10.020"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(22)02185-7"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201200112"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jare.2019.10.005"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.37201\/req\/128.2021"},{"key":"e_1_2_13_19_2","first-page":"51","article-title":"Documentation of Vancomycin-Resistant Staphylococcus aureus (VRSA) Among Children With Atopic Dermatitis in the Qassim Region, Saudi Arabia","volume":"21","author":"Alzolibani A. 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