{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T16:34:27Z","timestamp":1777048467740,"version":"3.51.4"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":113,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100002842","name":"Chiang Mai University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002842","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Malaria continues to be a significant global health burden, with over 249 million cases and 619,000 deaths reported in 2022, primarily affecting tropical and subtropical regions. The emergence of drug\u2010resistant strains of\n                    <jats:italic>Plasmodium falciparum<\/jats:italic>\n                    has reduced the efficacy of artemisinin\u2010based combination therapies (ACTs), necessitating the exploration of alternative treatment strategies. This study investigates the antimalarial efficacy of\n                    <jats:italic>Tinospora crispa<\/jats:italic>\n                    extract (TCE) and artesunate (ART) against\n                    <jats:italic>Plasmodium berghei<\/jats:italic>\n                    ANKA infection in ICR mice. Both ART and TCE demonstrated dose\u2010dependent antimalarial activity, with effective doses (ED\n                    <jats:sub>50<\/jats:sub>\n                    ) of 200.31\u2009mg\/kg and 1.96\u2009mg\/kg, respectively. The combination of ART and TCE exhibited synergistic effects, achieving significantly higher parasitemia inhibition compared to monotherapy, with combination index (CI) values\u2009&lt;\u20091 at ED\n                    <jats:sub>50<\/jats:sub>\n                    , ED\n                    <jats:sub>50\/2<\/jats:sub>\n                    , and ED\n                    <jats:sub>50\/4<\/jats:sub>\n                    . Furthermore, combination therapy provided enhanced protection against packed cell volume (PCV) reduction and body weight (BW) loss, along with prolonged mean survival time (MST) compared to untreated and single\u2010treatment groups. These findings highlight the potential of combining TCE with ART as a novel antimalarial strategy, offering synergistic benefits that may mitigate drug resistance and improve treatment outcomes. Further studies are warranted to explore the molecular mechanisms underlying these interactions and to evaluate the efficacy of this combination in clinical settings.\n                  <\/jats:p>","DOI":"10.1155\/jotm\/6681227","type":"journal-article","created":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T15:39:10Z","timestamp":1777045150000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Antimalarial Potency of\n                    <i>Tinospora crispa<\/i>\n                    Stem Extract and Artesunate Against\n                    <i>Plasmodium berghei<\/i>\n                    : A Study on Combination Therapy"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9067-2051","authenticated-orcid":false,"given":"Sakaewan","family":"Ounjaijean","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9774-2139","authenticated-orcid":false,"given":"Voravuth","family":"Somsak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_13_1_2","unstructured":"WHO World Malaria Report https:\/\/wwwmmvorg\/newsroom\/publications\/world-malaria-reports%202023."},{"key":"e_1_2_13_2_2","unstructured":"Cdc Malaria\u2019s Impact Worldwide https:\/\/wwwcdcgov\/malaria\/php\/impact\/indexhtml%202023."},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0140-6736(23)01249-7"},{"key":"e_1_2_13_4_2","unstructured":"UK A Malaria-Free World by 2030 https:\/\/amrefukorg\/news\/2024\/05\/world-malaria-day%202024."},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.2147\/idr.s405668"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/tropicalmed9090223"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/cmr.00109-24"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929867328666210329112354"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2017.06.013"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2016.00059"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0378-8741(98)00135-4"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.1155\/2019\/5464519"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.parint.2018.10.009"},{"key":"e_1_2_13_14_2","doi-asserted-by":"publisher","DOI":"10.1155\/2015\/738608"},{"key":"e_1_2_13_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2008.05.012"},{"key":"e_1_2_13_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12906-023-03850-y"},{"key":"e_1_2_13_17_2","doi-asserted-by":"publisher","DOI":"10.1080\/00034983.1975.11686997"},{"key":"e_1_2_13_18_2","doi-asserted-by":"publisher","DOI":"10.3390\/molecules24193601"},{"key":"e_1_2_13_19_2","doi-asserted-by":"publisher","DOI":"10.3923\/pjbs.2013.160.167"},{"key":"e_1_2_13_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/s0169-4758(00)01774-9"},{"key":"e_1_2_13_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/bf01695812"},{"key":"e_1_2_13_22_2","first-page":"102","article-title":"Studies on the Mechanism of Anemia in Rodent Malaria","volume":"4","author":"Wu Y. 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