{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T06:07:14Z","timestamp":1779257234530,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T00:00:00Z","timestamp":1779148800000},"content-version":"vor","delay-in-days":138,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100020905","name":"Universidad Surcolombiana","doi-asserted-by":"publisher","award":["3660"],"award-info":[{"award-number":["3660"]}],"id":[{"id":"10.13039\/501100020905","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Platelets are increasingly recognized as contributors to the cytokine storm in dengue, yet their residual presence in plasma may bias cytokine measurements. We compared MCP\u20101 levels between paired regular plasma (RP) and platelet\u2010poor plasma (PPP) in children with dengue and evaluated how depletion influences cytokine quantification.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We first conducted a systematic review of plasma isolation methods in pediatric dengue studies. We then collected paired RP and PPP from 16 hospitalized children with dengue (11 with warning signs and 5 with severe dengue). The PPP was obtained by double centrifugation and verified to contain &lt;\u200910,000 platelets\/\u03bcL. Absolute platelet counts were determined by flow cytometry using an antihuman CD41 antibody and bead\u2010based quantification. After one freeze\u2013thaw cycle, MCP\u20101 and IFN\u2010\u03b3 (negative control for platelet secretion) were measured by cytometric bead array.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Among 167 reviewed articles, only 40% reported plasma isolation methods and 5% performed platelet depletion, with wide variability in centrifugation protocols. In our cohort, 63% of RP samples exceeded the platelet\u2010poor threshold compared with only 10% of PPP after double\u2010spin depletion. After effective platelet removal, MCP\u20101 levels increased nearly 5\u2010fold in PPP versus RP (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.008), while IFN\u2010\u03b3 showed only a very modest rise, indicating that platelets are highly susceptible to physical and mechanical factors during the depletion process.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Standard plasma isolation fails to consistently remove platelets, and subsequent depletion protocols can artifactually elevate the platelet\u2010associated cytokines MCP\u20101. These findings underscore the need for standardized reporting of platelet depletion methods in dengue immunology research.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/jotm\/8409965","type":"journal-article","created":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T05:18:04Z","timestamp":1779254284000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Platelet Depletion Alters MCP\u20101 Measurements in Plasma From Children With Dengue"],"prefix":"10.1155","volume":"2026","author":[{"given":"H\u00e9ctor Felipe","family":"Ocampo-Amariles","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sebasti\u00e1n","family":"Castro-Trujillo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0129-5210","authenticated-orcid":false,"given":"Carlos F.","family":"Narv\u00e1ez","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,19]]},"reference":[{"key":"e_1_2_12_1_2","doi-asserted-by":"crossref","unstructured":"KrishnaG. 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