{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T13:30:45Z","timestamp":1773927045998,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,2]],"date-time":"2026-01-02T00:00:00Z","timestamp":1767312000000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["NSTC110-2320-B-038-064-MY3"],"award-info":[{"award-number":["NSTC110-2320-B-038-064-MY3"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["111-2327-B-006-005"],"award-info":[{"award-number":["111-2327-B-006-005"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["113-2320-B-038-027"],"award-info":[{"award-number":["113-2320-B-038-027"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["113-2327-B-006-002"],"award-info":[{"award-number":["113-2327-B-006-002"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020595","name":"National Science and Technology Council","doi-asserted-by":"publisher","award":["114-2320-B-038-060"],"award-info":[{"award-number":["114-2320-B-038-060"]}],"id":[{"id":"10.13039\/100020595","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Tropical Medicine"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Dengue virus (DENV) infection can potentially lead to severe dengue disease due to the risk of antibody\u2010dependent enhancement. This study reports a comparative analysis of the host cytokine\/chemokine response triggered by primary and secondary DENV infections using an artificial ex vivo whole\u2010blood coculture model to simulate viremia during the acute febrile phase of infection. Using ex vivo primary and secondary DENV infection modes, a dengue\u2010specific customized multiplex cytokine\/chemokine assay was employed. Secondary infection did not exacerbate DENV\u2010induced hematological and cytopathological changes, such as alterations in complete blood count, intracellular vacuolization, and thrombophagocytosis. However, cytokine\/chemokine assay revealed a significant increase in the production of MIP\u20101\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , MIP\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    , IL\u20106, TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , and RANTES. Notably, a substantial decrease in NS1 levels indicated the neutralization effect in individuals with prior DENV exposure or secondary infection group, especially in some cases of secondary infection. This was accompanied by pre\u2010existing anti\u2010E antibodies, highly associated with IL\u20106 overproduction. These findings support the potential strategy of assessing DENV susceptibility using NS1 and IL\u20106 using an ex vivo method.\n                  <\/jats:p>","DOI":"10.1155\/jotm\/9350179","type":"journal-article","created":{"date-parts":[[2026,1,3]],"date-time":"2026-01-03T05:13:49Z","timestamp":1767417229000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Profiling an Enhanced IL\u20106 Expression Distinguishes Host Susceptibility to Primary and Secondary Infections of the Dengue Virus in an Ex Vivo Whole\u2010Blood Coculture Model"],"prefix":"10.1155","volume":"2026","author":[{"given":"Josephine Diony","family":"Nanda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Kai","family":"Jhan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rahmat 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