{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T18:33:03Z","timestamp":1772994783157,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T00:00:00Z","timestamp":1755734400000},"content-version":"vor","delay-in-days":232,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Pregnancy"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    Amniocentesis (AC) remains the most commonly performed prenatal invasive diagnostic test. The data available till now have been collected before the era of high\u2010end ultrasound machines, NIPS, and chromosomal microarrays. In selected cases, whole\u2010exome sequencing is also offered prenatally. The evolution of ultrasound, NIPS, and genetic testing has made us revisit this topic.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Objective:<\/jats:bold>\n                    We aimed to research and revisit AC data regarding the indications, procedures, genetic testing methods, and outcomes. We reinforce the knowledge of AC, offer tips to minimize complications, and help communicate and counsel patients based on the AC data.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods:<\/jats:bold>\n                    It was a retrospective study from October 2019 to March 2023 in a tertiary care fetal medicine center in a university hospital. A total of 321 patients who underwent AC were analyzed. We observed the demographic details, indications, procedure details, and maternal\u2013fetal and neonatal outcomes.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Results:<\/jats:bold>\n                    During the study period, 321 patients underwent AC. Abnormal ultrasound findings (71%) were the most common indication for AC. Then, 9% (30\/321) had abnormal genetic results. Down syndrome was the most common abnormality (14), followed by Edwards syndrome. Then, 47.96% of cases were in age &gt; 35\u2009years. We had three cases of bloody tap, one leak per vagina, and two missed abortions following AC. Then, 58% had live births.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    AC is a relatively safe procedure, and even with the advent of NIPS, it remains the gold standard prenatal diagnostic genetic testing method. Major structural anomalies and parental chromosomal anomalies are irreplaceable indications of AC. The technique and expertise of health professionals dictate the complication rate of that center. Chromosomal microarray, DNA storage, and whole\u2010exome sequencing have added an extended armamentarium to our discovery of genetic diseases. Maternal and neonatal outcomes after AC are favorable, so do not hesitate to carry out this invasive test when indicated.\n                  <\/jats:p>","DOI":"10.1155\/jp\/9983529","type":"journal-article","created":{"date-parts":[[2025,8,22]],"date-time":"2025-08-22T06:50:51Z","timestamp":1755845451000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A Four\u2010Year Retrospective Study of Amniocentesis in a Tertiary Care Center in South India\u2014Lessons Learnt"],"prefix":"10.1155","volume":"2025","author":[{"given":"Jetti Gayatri","family":"Jahnavi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7503-2670","authenticated-orcid":false,"given":"Roopa Padavagodu","family":"Shivananda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akhila","family":"Vasudeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nivedita","family":"Hegde","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rashmi","family":"Natarajan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"e_1_2_9_1_2","volume-title":"Amniocentesis","author":"Jindal A.","year":"2023"},{"key":"e_1_2_9_2_2","first-page":"113","article-title":"A Four-year Retrospective Study Of Amniocentesis: One Centre Experience","volume":"12","author":"Daniilidis A.","year":"2008","journal-title":"Hippokratia"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/14651858.CD003252.pub2"},{"key":"e_1_2_9_4_2","unstructured":"Policy Priorities Current ACOG Guidance NIPT Summary of Recommendations 2024 https:\/\/www.acog.org\/advocacy\/policy-priorities\/non-invasive-prenatal-testing\/current-acog-guidance."},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.5212"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.31083\/j.ceog4806209"},{"key":"e_1_2_9_7_2","first-page":"260","article-title":"Amniocentesis and Chorionic Villus Sampling","volume":"159","author":"Shulman L. P.","year":"1993","journal-title":"Western Journal of Medicine"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/uog.14636"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31940-8"},{"key":"e_1_2_9_10_2","article-title":"The 11-13 Weeks Scan","author":"Nicolaides K. H.","year":"2004","journal-title":"Fetal Medicine Foundation"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.2637"},{"key":"e_1_2_9_12_2","doi-asserted-by":"crossref","unstructured":"American College of Obstetricians and Gynecologists Prenatal Diagnostic Testing for Genetic Disorders Obstetrics & Gynecology 2016 127 e108\u2013122.","DOI":"10.1097\/AOG.0000000000001405"},{"key":"e_1_2_9_13_2","unstructured":"Diagnostic Amniocentesis - UpToDate 2024 https:\/\/www.uptodate.com."},{"key":"e_1_2_9_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tjog.2021.05.018"},{"key":"e_1_2_9_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(86)91218-3"},{"key":"e_1_2_9_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/pd.1528"},{"key":"e_1_2_9_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1479-828X.1999.tb03397.x"},{"key":"e_1_2_9_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0223(200003)20:3<247::AID-PD794>3.0.CO;2-O"},{"key":"e_1_2_9_19_2","first-page":"239","article-title":"Amniocentesis-Related Adverse Outcomes According to Placental Location and Risk Factors for Fetal Loss After Midtrimester Amniocentesis","volume":"38","author":"Kalogiannidis I.","year":"2011","journal-title":"Clinical and Experimental Obstetrics & Gynecology"},{"key":"e_1_2_9_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e318162eb53"},{"key":"e_1_2_9_21_2","first-page":"117","volume-title":"Methods in Molecular Biology","author":"Buchovecky C. 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