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Phillyrins (PHNs) are lignan glycosides that have been reported to exhibit diverse biological activities, including potential anticancer effects. We aimed (1) to assess the effect of PHN on PC3 prostate cancer cells and (2) to examine cellular responses associated with its activity. To assess the effects of PHN, it was exposed to various concentrations of PHN (1, 2.5, and 5\u2009\u03bcM) for 24\u2009h. A wound\u2010healing assay was performed to evaluate cell migration. Western blotting and immunofluorescence were used to investigate the expression of adenosine monophosphate\u2013activated protein kinase (AMPK)\u2013associated proteins and transcription factors involved in epithelial\u2013mesenchymal transition (EMT). PHN exposure was associated with reduced migratory capacity of PC3 cells under noncytotoxic conditions. PHN exposure was associated with decreased \u03b1\u2010smooth muscle actin, Snail, and Slug expression while increasing E\u2010cadherin expression. Sirtuin 1 (SIRT1) and nuclear respiratory factor 1 (NRF1) protein levels were upregulated in PHN\u2010treated cells, accompanied by changes in AMPK protein expression. Pharmacological inhibition with Compound C attenuated PHN\u2010associated changes in EMT\u2010related marker expression in PC3 cells. Thus, PHN exposure may influence EMT\u2010associated phenotypes in PC3 cells, potentially involving AMPK\u2010associated signaling.<\/jats:p>","DOI":"10.1155\/jt\/2022957","type":"journal-article","created":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T08:56:43Z","timestamp":1775897803000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Phillyrin Modulates AMPK\u2010Associated Cellular Responses and Migration in PC3 Prostate Cancer Cells"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5044-1646","authenticated-orcid":false,"given":"Cheng-Hsin","family":"Lu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Hsien","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pei-Fang","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard Chen-Yu","family":"Wu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1352-6680","authenticated-orcid":false,"given":"Hsing-Chia","family":"Mai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei-Lun","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sih-Han","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chien-Ming","family":"Lai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0075-7014","authenticated-orcid":false,"given":"Victor C.","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4782-9697","authenticated-orcid":false,"given":"Chiang-Ting","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,11]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21708"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2016.05.043"},{"key":"e_1_2_13_3_2","unstructured":"Taiwanese Health Promotion AdministrationandMinistry of Health and Welfare Cancer Registry Annual Report 2020 https:\/\/www.hpa.gov.tw\/file\/attach\/16434\/file_20339.pdf."},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajur.2014.08.007"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2014.2874"},{"key":"e_1_2_13_6_2","first-page":"2377","article-title":"Effect of Forsythin in Inhibiting Renal Cell Carcinoma via PI3K\/Akt Signaling Pathway","volume":"50","author":"Wu L. 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