{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T09:49:50Z","timestamp":1791884990507,"version":"4.3.4"},"reference-count":21,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:00:00Z","timestamp":1791849600000},"content-version":"vor","delay-in-days":285,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Journal of Toxicology"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Vitex negundo<\/jats:italic>\n                      and\n                      <jats:italic>Zingiber officinale<\/jats:italic>\n                      are predominantly used in day\u2010to\u2010day life as food or supplements. Both are being reported individually for therapeutic properties. A food supplement consisting of leaves and twigs of\n                      <jats:italic>Vitex negundo<\/jats:italic>\n                      and rhizome of\n                      <jats:italic>Zingiber officinale<\/jats:italic>\n                      , the first\u2010of\u2010a\u2010kind proprietary blend extract (MUVZ), was developed for joint health.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      As part of the safety profile, a subchronic toxicity study was performed in Sprague Dawley rats under compliance with Good Laboratory Practice (GLP). The study aimed to determine the potential toxicity of MUVZ following oral (gavage) administration by following Organisation for Economic Co\u2010operation and Development (OECD) Guideline 408. MUVZ was administered for 90\u2009days, with a recovery period of 28\u2009days. MUVZ was administered at 0 (G1), 1000 (G2), 2000 (G3) and 3000 (G4) mg.kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      body weight (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910\/sex\/group); vehicle control (G1R) and high\u2010dose recovery groups 3000\u2009mg.kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      \u00b7b.w (G4R) (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u20095\/sex\/group).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>No MUVZ\u2010related effects were observed in mortality, morbidity, detailed clinical signs, body weight and feed consumption. Statistical significance was found in levels of clinical pathology parameters; however, there was no dose dependency, and further correlative histopathological findings were observed. At the end of the experimental trial, no test article\u2013induced gross and microscopic pathological lesions were observed across the treatment groups.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Based on the findings, no toxicologically significant findings were derived at the highest dose of 3000\u2009mg.kg\n                      <jats:sup>\u22121<\/jats:sup>\n                      body weight.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/jt\/9540593","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T09:40:22Z","timestamp":1791884422000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["90\u2010Day Repeated\u2010Dose Toxicological Assessment of MUVZ in Sprague Dawley Rats: Subchronic Study"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2650-6395","authenticated-orcid":false,"given":"Devanand","family":"Shanmugasundaram","sequence":"first","affiliation":[{"name":"Compliance and Operations (Toxicology) Vedic Lifesciences Pvt. 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