{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,11]],"date-time":"2026-07-11T18:23:37Z","timestamp":1783794217127,"version":"3.55.0"},"reference-count":26,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T00:00:00Z","timestamp":1774656000000},"content-version":"vor","delay-in-days":86,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFC3500403"],"award-info":[{"award-number":["2022YFC3500403"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Diminished ovarian reserve (DOR) is a major challenge in reproductive medicine, especially with delayed childbearing. Current treatments show limited efficacy and side effects. Electroacupuncture (EA), a multitarget nonpharmacological therapy, may protect ovarian function and regulate immune balance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study investigated the protective effects of EA and its potential mechanisms in cyclophosphamide (CTX)\u2010induced DOR in rats, with a focus on apoptosis\u2010related changes in granulosa cells and T helper 17 (Th17)\/regulatory T (Treg)\u2010related immune modulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A CTX\u2010induced DOR rat model was treated with EA at CV4 (Guanyuan) and CV6 (Qihai), with sham EA and normal groups as controls. Estrous cycle, ovarian indices, serum hormones, and cytokines were assessed. Ovarian morphology, follicle counts, apoptosis, and protein expression were evaluated by histology, TUNEL, western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF). Splenic Treg\/Th17 cells were analyzed by flow cytometry, and RNA sequencing identified EA\u2010regulated pathways.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>EA improved estrous cyclicity, ovarian morphology, and follicular development; improved follicle\u2010associated marker expression; and reduced elevated FSH and LH levels in CTX\u2010induced DOR rats. AMH and E2 showed upward trends after EA treatment, but these changes did not reach statistical significance. EA reduced TUNEL positivity, restored Ki67 expression, and favorably regulated the Bcl\u20102\/Bax axis, while cleaved caspase\u20103 remained elevated and was not significantly altered. EA was also associated with normalization of Th17\/Treg\u2010related immune indices in the spleen, serum, and ovary, including reduced IL\u20106, IL\u201017A, IL\u20101\u03b2, TNF\u2010\u03b1, and ROR\u03b3t, and increased FOXP3, IL\u201010, and TGF\u2010\u03b21. Transcriptomic analysis revealed enrichment of immune\u2010related pathways, consistent with the functional findings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>EA ameliorated CTX\u2010induced ovarian injury and was associated with improved follicular development, attenuation of apoptosis\u2010related changes, and Th17\/Treg\u2010related immune modulation. These findings support a potential ovarian\u2010protective and immunoregulatory role of EA in DOR, although further studies are required to verify endocrine efficacy and mechanistic causality.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/mi\/1067286","type":"journal-article","created":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T11:12:36Z","timestamp":1774696356000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Electroacupuncture Ameliorates Cyclophosphamide\u2010Induced Ovarian Impairment in Rats With Diminished Ovarian Reserve and is Associated With Th17\/Treg\u2010Related Immune Modulation"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9815-2026","authenticated-orcid":false,"given":"Xiaoyu","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4987-7927","authenticated-orcid":false,"given":"Zhanyu","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-3549-6628","authenticated-orcid":false,"given":"Ruixin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-3113-0083","authenticated-orcid":false,"given":"Zhengqi","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5901-7229","authenticated-orcid":false,"given":"Kexiang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-8951-8697","authenticated-orcid":false,"given":"Yuxia","family":"Ma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,28]]},"reference":[{"key":"e_1_2_13_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2024.09.038"},{"key":"e_1_2_13_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2015.05.017"},{"key":"e_1_2_13_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2023.138727"},{"key":"e_1_2_13_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2024.04.229"},{"key":"e_1_2_13_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10815-017-1058-4"},{"key":"e_1_2_13_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12958-018-0343-0"},{"key":"e_1_2_13_7_2","doi-asserted-by":"publisher","DOI":"10.5582\/ddt.2022.01109"},{"key":"e_1_2_13_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2025.103614"},{"key":"e_1_2_13_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s43032-024-01682-w"},{"key":"e_1_2_13_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.133994"},{"key":"e_1_2_13_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jri.2024.104295"},{"key":"e_1_2_13_12_2","doi-asserted-by":"publisher","DOI":"10.2147\/IJWH.S455733"},{"key":"e_1_2_13_13_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13048-023-01296-x"},{"key":"e_1_2_13_14_2","article-title":"Research Progress on the Relationship Between Th17\/Treg Imbalance and Ovarian Function Decline","volume":"18","author":"Yapeng Han B. 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