{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T10:07:52Z","timestamp":1784023672968,"version":"3.55.0"},"reference-count":58,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,14]],"date-time":"2026-07-14T00:00:00Z","timestamp":1783987200000},"content-version":"vor","delay-in-days":194,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82202753"],"award-info":[{"award-number":["82202753"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>NLRP3 inflammasome plays a pivotal role in the pathogenesis of intervertebral disc (IVD) degeneration (IDD). This study aimed to identify NLRP3 inflammasome\u2010related biomarkers in IDD and analyze their functions.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>First, the differentially expressed genes (DEGs) (IDD vs. control) in GSE124272 were detected. The NLRP3 inflammasome\u2010related genes (NIRGs) scores for all samples were calculated using single\u2010sample gene set enrichment analysis (ssGSEA). Subsequently, the key module with the strongest correlation with NIRGs scores was selected using weighted gene coexpression network analysis (WGCNA). Biomarkers were defined as the DEGs and genes showing consistent expression trends across both the GSE124272 and GSE150408 datasets. Function enrichment and immune infiltration were analyzed. The abundance of the key biomarkers was validated by real\u2010time quantitative PCR (RT\u2010qPCR). To explore the effect of core targets on IDD by constructing IDD in vitro model.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      About 1608 intersection genes were derived from overlapping the above 2123 DEGs and 10695 key module genes. Subsequently, the top 10 genes were identified. Based on gene expression analysis, disheveled, EGL\u201010, and pleckstrin domain\u2010containing 1 (DEPDC1), and Opa interacting protein 5 (OIP5) were selected as biomarkers. Functional enrichment analysis revealed that these biomarkers were primarily enriched in cytosolic ribosomes and DNA replication. Immune infiltration analysis identified 13 differential immune cells. Endothelial cells were inversely correlated with DEPDC1, whereas conventional dendritic cells (CDC) demonstrated a pronounced positive relationship with OIP5. RT\u2010qPCR results confirmed that the expression of OIP5 and DEPDC1 in IDD patients was downregulated by more than 50% (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.05). Cell experiments showed that up\u2010regulation of OIP5 led to a 2\u2010fold reduction in cell proliferation (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001) and a three\u2010fold increase in apoptosis in nucleus pulposus (NP) cells derived from IDD patients (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Taken together, OIP5 has been identified as a peripheral blood biomarker linked to the NLRP3 inflammasome and may be involved in the pathogenesis of IDD.<\/jats:p>\n                  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