{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T17:28:28Z","timestamp":1770744508806,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,2,9]],"date-time":"2006-02-09T00:00:00Z","timestamp":1139443200000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Dutch government","award":["BSIK03016"],"award-info":[{"award-number":["BSIK03016"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p>Inflammatory processes are known to be involved at least in the\nearly phase of complex regional pain syndrome type 1 (CRPS1).\nBlister fluid obtained from the involved extremities displayed\nincreased amounts of proinflammatory cytokines IL\u20106 and TNF<jats:italic>\u03b1<\/jats:italic>\n compared with the noninvolved extremities. The aim of this paper\nis to investigate the involvement of mediators by measurement of\nseveral other cytokines using new detection techniques that enable\nmultiple cytokine measurement in small samples. The use of a\nmultiplex\u201025 bead array cytokine assay and Luminex technology\nenabled simultaneous measurement of representative (1)\nproinflammatory cytokines such as GM\u2010CSF, IL\u20101<jats:italic>\u03b2<\/jats:italic>, IL\u20101RA,\nIL\u20106, IL\u20108, and TNF\u2010<jats:italic>\u03b1<\/jats:italic>; (2) Th1\/Th2 distinguishing\ncytokines IFN\u2010<jats:italic>\u03b3<\/jats:italic>, IL\u20102, IL\u20102R, IL\u20104, IL\u20105, and IL\u201010; (3)\nnonspecific acting cytokines IFN\u2010<jats:italic>\u03b1<\/jats:italic>, IL\u20107, IL\u201012p40\/p70,\nIL\u201013, IL\u201015, and IL\u201017; and (4) chemokines eotaxin, IP\u201010, MCP\u20101,\nMIP\u20101<jats:italic>\u03b1<\/jats:italic>, MIP\u20101<jats:italic>\u03b2<\/jats:italic>, MIG, and RANTES. Although minimal\ndetection levels are significantly higher in the bead array system\nthan those in common ELISA assays, in blister fluid, IL\u20101RA, IL\u20106,\nIL\u20108, TNF\u2010<jats:italic>\u03b1<\/jats:italic>, IL\u201012p40\/p70, MCP\u20101, and MIP\u20101<jats:italic>\u03b2<\/jats:italic> were\ndetectable and increased in CRPS1 affected extremities. Levels of\nIL\u20106 and TNF\u2010<jats:italic>\u03b1<\/jats:italic> simultaneously measured by ELISA (Sanquin\nCompact kit) and by multiplex\u201025 bead array assay (Biosource) were\nhighly correlated (<jats:italic>r<\/jats:italic> = 0.85, <jats:italic>P<\/jats:italic> &lt; .001\n for IL\u20106 and <jats:italic>r<\/jats:italic> = 0.88,\n\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t<jats:italic>P<\/jats:italic> &lt; .001 for TNF\u2010<jats:italic>\u03b1<\/jats:italic>). Furthermore, IP\u201010 and eotaxin were\ndetectable but diminished in CRPS1, whereas detectable amounts of\nIL\u201010 were similar in involved and noninvolved extremities.\nMultiplex bead array assays are useful systems to establish the\ninvolvement of cytokines in inflammatory processes by measurements\nin blister fluids of CRPS1. Ten representative cytokines were\ndetectable. However, detection levels and amounts measured are at\nleast 3 times higher in the multiplex\u201025 array assay than in the\nELISA assays used simultaneously for the measurement of cytokines.<\/jats:p>","DOI":"10.1155\/mi\/2006\/28398","type":"journal-article","created":{"date-parts":[[2006,4,5]],"date-time":"2006-04-05T12:45:21Z","timestamp":1144241121000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":112,"title":["Multiplex Bead Array Assay for Detection of 25 SolubleCytokines in Blister Fluid of Patients with ComplexRegional Pain Syndrome Type 1"],"prefix":"10.1155","volume":"2006","author":[{"given":"Claudia","family":"Heijmans-Antonissen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Feikje","family":"Wesseldijk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Renate JM","family":"Munnikes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank JPM","family":"Huygen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"van der Meijden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wim C. 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