{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T10:27:08Z","timestamp":1783852028897,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,11,9]],"date-time":"2006-11-09T00:00:00Z","timestamp":1163030400000},"content-version":"vor","delay-in-days":312,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Secretar\u00eda de Educaci\u00f3n Publica","award":["UAEMOR-PTC-02-01"],"award-info":[{"award-number":["UAEMOR-PTC-02-01"]}]},{"name":"Programa de Mejoramiento del Profesorado","award":["UAEMOR-PTC-02-01"],"award-info":[{"award-number":["UAEMOR-PTC-02-01"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:p>CSV consists of a very complex of molecules and demonstrates significant cellular activities capable of stimulating immune functions in vivo. The purpose of this study was to analyze the effects of CSV on sex, weight, route of injection and the balance of pro\u2010 and anti\u2010inflammatory cytokines in mice. The susceptibility and route of injection were analyzed by lethal (LD<jats:sub>50<\/jats:sub>) determination. The effects of CSV were also analyzed in blood from immunized mice using detection by means of antibodies and mediators production. Several functional bioassays were employed: TNF activity was assayed by measuring its cytotoxic activity in L929 cells, and other cytokines were assayed by enzyme\u2010linked immunosorbent assay, whereas nitric oxide levels were detected by Griess colorimetric reactions in sera from BALB\/c mice. After injecting subcutaneously, the LD<jats:sub>50<\/jats:sub> presented an increase of the CSV correlation and similar levels of susceptibility were obtained for female and male from BALB\/c mice. Significant differences were observed in the time\u2010course of cytokine levels. The balance of pro\u2010 and anti\u2010inflammatory cytokines TNF\/IL\u201010 and IL\u20106\/IL\u201010 ratios were significantly higher in injected mice group when compared with those obtained for non\u2010injected group. The CSV is poor in antigenic composition and it is difficult to get antibodies specific to neutralizing the lethal factor. The effect of immunization with 0.5 LD<jats:sub>50<\/jats:sub> of CSV on the balance of pro\u2010 and anti\u2010inflammatory cytokines was measured. The maximum levels of TNF and IL\u20106, IFN\u2010<jats:italic>\u03b3<\/jats:italic> and NO were observed on days 7 and 21 after immunization, respectively. IL\u201010 levels peaked between days 21 and 28 after immunization with CSV. With respect, to balance of pro\u2010 and anti\u2010inflammatory cytokines it was possible to observe that negative correlation between serum levels of IL\u20106\/IL\u201010 and TNF\/IL\u201010 exists. These ratios may possibly reflect the balance of pro\u2010 and anti\u2010inflammatory cytokines in serum, which may by manifested in the inflammatory status during the envenoming processes. In conclusion, an increase in the serum levels of TNF and IL\u20106 may be a useful marker for scorpion envenomation.<\/jats:p>","DOI":"10.1155\/mi\/2006\/54273","type":"journal-article","created":{"date-parts":[[2006,11,16]],"date-time":"2006-11-16T00:58:50Z","timestamp":1163638730000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Balance Between Pro\u2010 and Anti\u2010Inflammatory Cytokines in Mice Treated With <i>Centruroides noxius<\/i> Scorpion Venom"],"prefix":"10.1155","volume":"2006","author":[{"given":"Vera L.","family":"Petricevich","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,11,9]]},"reference":[{"key":"e_1_2_1_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(94)90384-0"},{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00251-7"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.2174\/1568010043343642"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01711538"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80533-6"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001303"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(00)80013-6"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(95)00182-4"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01877210"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.2.547"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.177.4.1205"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.152.2.7633696"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/clin.1997.4420"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.7.1185"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1368"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.2174\/0929866023408779"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","unstructured":"Committe Members. 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