{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:13:51Z","timestamp":1790633631615,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T00:00:00Z","timestamp":1762819200000},"content-version":"vor","delay-in-days":314,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2021MH079"],"award-info":[{"award-number":["ZR2021MH079"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Due to the complex pathogenesis of sequelae of pelvic inflammatory disease (SPID), targeted therapeutic agents are still lacking. Here, we investigated the interactions between neutrophils and uterine fibroblasts (FBs) in developing tissue fibrosis in SPID.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A rat model of SPID was constructed to assess the roles of autophagy and neutrophil extracellular traps (NETs) in SPID rats. Single\u2010cell sequencing data from the public database GSE223639 were utilized to identify the specific cell cluster FBs where NETs act. A DMSO\u2010induced HL\u201060\u2010driven neutrophil\u2010like (dHL\u201060) cell model was established, and neutrophil\u2010like cells were treated with rapamycin and MHY1485 to activate and inhibit autophagy, respectively, to observe the differences in the production of NETs. NETs were cocultured with FBs to observe the effects on FB proliferation, migration, apoptosis, and phenotypic transformation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In vivo experiments revealed that there was a consistency in the expression of autophagy and NETs in the adherent tissues of rats with the SPID model and that autophagy promotes the generation of NETs, which are collectively involved in the fibrosis of pelvic tissues in SPID. Single\u2010cell sequencing identified FBs, the cells in which NETs play a major role in aseptic inflammation. Further in vitro studies confirmed that NETs inhibit FB apoptosis while promoting horizontal and longitudinal migration, phenotypic transformation, and hyperproliferation of FBs, thereby exacerbating tissue fibrosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Autophagy promotes the generation of NETs, which facilitates FB transformation and hyperproliferation and exacerbates the degree of adhesion and fibrosis in the pelvic tissue of SPID.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/mi\/3113542","type":"journal-article","created":{"date-parts":[[2025,11,12]],"date-time":"2025-11-12T07:21:13Z","timestamp":1762932073000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Neutrophil\u2010Fibroblast Crosstalk Drives Immunofibrosis in Sequelae of Pelvic Inflammatory Disease Through Neutrophil Extracellular Traps"],"prefix":"10.1155","volume":"2025","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8145-8774","authenticated-orcid":false,"given":"Chunxiao","family":"Dang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinxing","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-9796-9510","authenticated-orcid":false,"given":"Xiao","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2269-6788","authenticated-orcid":false,"given":"Xian","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,11,11]]},"reference":[{"key":"e_1_2_15_1_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00404-019-05272-z"},{"key":"e_1_2_15_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31821f918c"},{"key":"e_1_2_15_3_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25126460"},{"key":"e_1_2_15_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.29914"},{"key":"e_1_2_15_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.41062"},{"key":"e_1_2_15_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-020-00103-1"},{"key":"e_1_2_15_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2018-213181"},{"key":"e_1_2_15_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20201012"},{"key":"e_1_2_15_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01252"},{"key":"e_1_2_15_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2010.150"},{"key":"e_1_2_15_11_2","doi-asserted-by":"publisher","DOI":"10.34133\/research.0050"},{"key":"e_1_2_15_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/burnst\/tkad017"},{"key":"e_1_2_15_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/burnst\/tkae043"},{"key":"e_1_2_15_14_2","unstructured":"YuX. 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