{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T20:31:49Z","timestamp":1787603509307,"version":"build-2736575974"},"reference-count":33,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T00:00:00Z","timestamp":1737244800000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,1,1]],"date-time":"2025-01-01T00:00:00Z","timestamp":1735689600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100018556","name":"Science and Technology Program of Suzhou","doi-asserted-by":"publisher","award":["SKY2022051"],"award-info":[{"award-number":["SKY2022051"]}],"id":[{"id":"10.13039\/501100018556","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81970027"],"award-info":[{"award-number":["81970027"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018636","name":"Xuzhou Science and Technology Program","doi-asserted-by":"publisher","award":["KC22196"],"award-info":[{"award-number":["KC22196"]}],"id":[{"id":"10.13039\/501100018636","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    <jats:bold>Background:<\/jats:bold>\n                    This study aimed to explore the mechanisms underlying T\u2010cell differentiation in asthma.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Methods and Results:<\/jats:bold>\n                    Flow cytometry was performed to detect Th cells. LC\u2010MS\/MS was performed to assess lipid metabolism. HE staining was performed to assess the pathological changes of the lung tissues. ELISA was performed to detect cytokine levels. The results of quantitative real\u2010time polymerase chain reaction (qRT\u2010PCR) and western blot showed that miR\u2010192\u20105p expression was decreased, while SCD1 expression was increased in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells isolated from the peripheral blood of children with asthma. The dual luciferase reporter assay determined the direct interaction between miR\u2010192\u20105p and SCD1. MiR\u2010192\u20105p inhibitor reduced ASCL3 and PPAR\u03b1, increased FASN and SREBP1c mRNA expression and protein levels in mouse spleen CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, and elevated Th2 and Th17 cells, but these effects were reversed by the SCD1 inhibitor. Oleic acid (OA) reduced Th1 cells and increased Th2 and Th17 cells in mouse spleen CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells treated with an SCD1 inhibitor. Additionally, pri\u2010miR\u2010192\u20105p expression was increased in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells isolated from the peripheral blood of asthmatic children, and the deletion of METTL3 upregulated pri\u2010miR\u2010192\u20105p expression in an m6A\u2010dependent manner. MiR\u2010192\u20105p mimic and inhibitor both reversed miR\u2010192\u20105p and SCD1 expression affected by overexpression or deletion of METTL3, both\n                    <jats:italic>in vivo<\/jats:italic>\n                    and\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Furthermore, METTL3 overexpression attenuated lung inflammation, elevated Th1 cells, and reduced Th2 and Th17 cells in CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells isolated from the peripheral blood of asthmatic mice. These effects were reversed by the miR\u2010192\u20105p inhibitor.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>Conclusion:<\/jats:bold>\n                    These results suggest that METTL3\/miR\u2010192\u20105p\/SCD1 axis regulates lipid metabolism and affects T cell differentiation, thus affecting asthma progression. This study may provide novel insights into the pathogenesis of asthma and a new treatment strategy.\n                  <\/jats:p>","DOI":"10.1155\/mi\/4955849","type":"journal-article","created":{"date-parts":[[2025,1,19]],"date-time":"2025-01-19T08:49:06Z","timestamp":1737276546000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["METTL3\/miR\u2010192\u20105p\/SCD1 Axis Regulates Lipid Metabolism to Affect T Cell Differentiation in Asthma"],"prefix":"10.1155","volume":"2025","author":[{"given":"Zhengrong","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dingwei","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suyu","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiyi","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinxing","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heting","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8602-3446","authenticated-orcid":false,"given":"Li","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,1,19]]},"reference":[{"key":"e_1_2_11_1_2","first-page":"1823","article-title":"Prevalence of Asthma and Its Management: A Review","volume":"68","author":"Rehman A.","year":"2018","journal-title":"JPMA. The Journal of the Pakistan Medical Association"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.11.025"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.anai.2016.04.022"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2020.05.003"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00251-015-0881-y"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0803560"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0905063106"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1177\/0300060518789819"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2019.109692"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.853848"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.09.032"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.alit.2022.05.002"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00394-018-1691-4"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep10222"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature14281"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2014.05.010"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2020.158853"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1155\/2021\/6634532"},{"key":"e_1_2_11_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.877650"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.1194\/jlr.M081836"},{"key":"e_1_2_11_21_2","first-page":"172","article-title":"Upregulation of SCD1 Gene-Related Lipid Synthesis by IL-6 Stimulus in HepG2 Cells in Vitro","volume":"22","author":"Baige C.","year":"2019","journal-title":"Journal of Practical Hepatology"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201804043"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsomega.1c01626"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1039\/D1CC04091J"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13010040"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-019-0168-5"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.28885"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.26355\/eurrev_202004_20817"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2016.11.017"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature23450"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2014.04.015"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08332-9"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201948257"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/mi\/4955849","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/mi\/4955849","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/mi\/4955849","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T22:17:56Z","timestamp":1773008276000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/mi\/4955849"}},"subtitle":[],"editor":[{"given":"Raffaele","family":"Capasso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2025,1]]},"references-count":33,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.1155\/mi\/4955849"],"URL":"https:\/\/doi.org\/10.1155\/mi\/4955849","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1]]},"assertion":[{"value":"2024-09-26","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-10","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-01-19","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"4955849"}}