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While microglial M1 polarization and pyroptosis are key drivers, their upstream regulators are incompletely understood. This study investigated the role of the ubiquitin ligase tripartite motif\u2010containing protein 14 (TRIM14) in regulating neuroinflammation following SCI. Using rat SCI models and BV2 microglia exposed to lipopolysaccharide (LPS), we assessed TRIM14 expression and its functional impact via knockdown and overexpression, alongside pharmacological neurofilament (NF)\u2010\u03baB inhibition (pyrrolidine dithiocarbamate [PDTC]). TRIM14 was upregulated in injured spinal cords and microglia, associated with injury severity. TRIM14 knockdown in microglia stabilized I\u03baB\u03b1 by inhibiting its ubiquitination, thereby suppressing NF\u2010\u03baB activation, M1 polarization, and NLRP3\u2010mediated pyroptosis. Conversely, TRIM14 overexpression exacerbated inflammation, effects markedly reversed by PDTC. In SCI rats, intralesional AAV\u2010CRISPR\/CasRx\u2010mediated TRIM14 silencing significantly attenuated neuroinflammation and neuronal apoptosis, enhanced axonal regeneration, and improved locomotor function. Mechanistically, TRIM14 knockdown suppressed NF\u2010\u03baB\/NLRP3 signaling, promoting a prorepair microenvironment. These results identify TRIM14 as a critical regulator of microglial activation and pyroptosis post\u2010SCI, suggesting its therapeutic targeting could be a viable strategy to promote neural repair.<\/jats:p>","DOI":"10.1155\/mi\/5053685","type":"journal-article","created":{"date-parts":[[2025,11,10]],"date-time":"2025-11-10T06:17:01Z","timestamp":1762755421000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["TRIM14 Inhibition Suppresses Microglial Polarization and Pyroptosis Through the NF\u2010\u03baB\/NLRP3 Pathway to Enhance Spinal Cord Injury 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