{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T11:08:54Z","timestamp":1774264134164,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":81,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["L246006"],"award-info":[{"award-number":["L246006"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005089","name":"Beijing Municipal Natural Science Foundation","doi-asserted-by":"publisher","award":["L254086"],"award-info":[{"award-number":["L254086"]}],"id":[{"id":"10.13039\/501100005089","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Platelet\u2013leukocyte aggregates (PLAs) drive immunothrombosis by promoting leukocyte activation and tissue factor release in sepsis. However, their utility as early predictors of sepsis\u2010induced coagulopathy (SIC) and disseminated intravascular coagulation (DIC) remains unestablished. This study aimed to develop and validate a PLA\u2010based model for SIC diagnosis and DIC prediction.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A prospective prediction study was conducted with 101 sepsis patients in Beijing Jishuitan Hospital between May 2024 and May 2025. Percentage and mean fluorescence intensity (MFI) of different subtypes of PLAs were measured by flow cytometry. Receiver operating characteristic (ROC) curves and Cox regression were performed, assessing the performance of PLAs to diagnose SIC and predict DIC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Platelet\u2013neutrophil aggregate MFI (PNA\u2010MFI) demonstrated excellent diagnostic accuracy for SIC (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001, AUC\u2009=\u20090.789, 95% CI: 0.699\u20130.880, cut\u2010off value\u2009=\u20092797, sensitivity\u2009=\u200976.2%, specificity\u2009=\u200978.0%, negative predictive value (NPV)\u2009=\u200983.9%, and positive predictive value (PPV)\u2009=\u200968.8%) and superior DIC prediction (\n                      <jats:italic>p<\/jats:italic>\n                      &lt; 0.001, AUC\u2009=\u20090.781, 95% CI: 0.680\u20130.883, cut\u2010off value\u2009=\u20092621, sensitivity\u2009=\u200983.3%, specificity\u2009=\u200964.9%, NPV\u2009=\u200992.6%, and PPV\u2009=\u200942.6%). Kaplan\u2013Meier analysis showed that high PNA\u2010MFI patients (PNA\u2010MFI\u2009\u2265\u20092621) were more likely to develop DIC (log\u2010rank\n                      <jats:italic>p<\/jats:italic>\n                      \u2009 &lt; 0.001, hazard ratio: 6.80 [high PNA\u2010MFI\/low PNA\u2010MFI], and 95% CI:2.97\u201315.61).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>PNA\u2010MFI is a promising biomarker for SIC diagnosis and DIC prediction. While internally validated, external validation is essential prior to clinical implementation, and further multicenter studies are warranted to definitively establish its clinical value.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/mi\/5580762","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T10:25:27Z","timestamp":1774261527000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Circulating Platelet\u2013Neutrophil Aggregates as Novel Biomarker for Coagulopathy Diagnosis and Disseminated Intravascular Coagulation Prediction in 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