{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T07:11:19Z","timestamp":1783926679653,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T00:00:00Z","timestamp":1783814400000},"content-version":"vor","delay-in-days":192,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074357"],"award-info":[{"award-number":["82074357"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Evidence has shown that acupuncture treatment could significantly reduce the activation inflammation in asthmatic mice. However, the underlying mechanism through which acupuncture influences asthma has not yet been elucidated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      OVA\u2010induced asthma in NLRP3\n                      <jats:sup>\u2012\/\u2012<\/jats:sup>\n                      and wild\u2010type C57BL mice, and lipopolysaccharides (LPS) induced inflammation in 16HBE cell were established in vivo and in vitro. H&amp;E, Masson, and Sirius staining, along with ELISA kits, Western Blot, PCR, lactate dehydrogenase (LDH), immunohistochemistry, and immunofluorescence techniques were employed to assess the miR\u2010223\/NLRP3\u2010mediated pyroptosis pathway in mitigating asthma\u2010induced inflammation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In vitro, acupuncture treatment alleviated airway inflammation in asthmatic mice, upregulated miR\u2010223 expression, inhibited the genes and proteins expression level in NLRP3 pathway, such as NLRP3, GSDMD, ASC, and Caspase\u20101, it also lowered the presence of cytokines tied to T cells. Activation of the NLRP3 pathway seemed to make asthma symptoms worse, whereas acupuncture treatment effectively alleviated the inflammation by modulating the miR\u2010223\/NLRP3 pathway. In vivo, after upregulating or downregulating miR\u2010223 expression in an LPS\u2010induced cell inflammation model, the expression levels of the NLRP3 pathway changed significantly. The dual\u2010luciferase assay revealed the specific interaction of miR\u2010223 to the NLRP3 gene, thereby suppressing its transcription.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>miR\u2010223\u20103p can bind to NLRP3 and suppresses the expression of NLRP3 gene. Acupuncture treatment can activate miR\u2010223\/NLRP3\u2010related pyroptosis pathway to regulate T\u2010lymphocyte immunity and effectively reduce airway inflammation in asthmatic mice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/mi\/5665377","type":"journal-article","created":{"date-parts":[[2026,7,13]],"date-time":"2026-07-13T06:53:03Z","timestamp":1783925583000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Acupuncture Treatment Alleviating Pyroptosis on Asthma Inflammation in Mice via Micro\u2010RNA\u2010223\/NLRP3 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