{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T10:50:34Z","timestamp":1775645434967,"version":"3.50.1"},"reference-count":53,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":97,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Recent studies suggest that alleviating pyroptosis may be an effective strategy for treating inflammation. This research explores the changes in cellular composition and pyroptotic pathways in dental pulp tissue during pulpitis and preliminarily validates the regulatory role of EZH2 in lipopolysaccharide (LPS)\u2010induced pyroptosis of vascular endothelial cells (ECs) via NLRP6, aiming to provide a theoretical foundation for understanding pulpitis mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Bioinformatics analysis of public single\u2010cell RNA sequencing (scRNA\u2010seq) and microarray data, together with RNA\u2010seq of EZH2\n                      <jats:sup>fl\/fl<\/jats:sup>\n                      ;Tie2\u2010Cre\n                      <jats:sup>+\/\u2212<\/jats:sup>\n                      pulp tissue, was performed to investigate the involvement of ECs and pyroptosis in pulpitis. Functional validation was conducted using an EZH2\u2010inhibited inflammatory model and an NLRP6 knockdown model.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Bioinformatics analysis revealed a strong association between pulpitis and EC pyroptosis. RNA\u2010seq further demonstrated that EZH2 deficiency was associated with enhanced pyroptotic responses in ECs. In vitro, EZH2 inhibition or NLRP6 knockdown markedly attenuated LPS\u2010induced inflammasome activation and pyroptosis in EOMA cells. This illustrates that the EZH2\/NLRP6 axis plays a critical regulatory role in EC pyroptosis and suggests its potential as a therapeutic target in pulpitis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>In summary, this study establishes ECs as key regulators in pulpitis inflammation, highlighting EZH2\u2019s role in modulating NLRP6 and the potential mechanism of pyroptosis in ECs.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/mi\/5876995","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T09:57:33Z","timestamp":1775642253000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["EZH2\u2010Mediated Epigenetic Modifications Induce Pyroptosis in Dental Pulp Endothelial Cells via Activation of NLRP6 Inflammasome During Pulpitis 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