{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T11:11:04Z","timestamp":1778929864980,"version":"3.51.4"},"reference-count":44,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T00:00:00Z","timestamp":1778457600000},"content-version":"vor","delay-in-days":130,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:p>\n                    Zhuye Shigao Decoction (ZSD), a classic traditional Chinese medicine (TCM) formula, has demonstrated therapeutic efficacy in clinical settings for acute pneumonia; however, its mechanism of action remains elusive. This study aims to investigate the therapeutic effects and potential mechanisms of ZSD in lipopolysaccharide (LPS)\u2010induced acute pneumonia in mice. A liquid chromatography\u2010mass spectrometry (LC\u2010MS)\u2010based metabolomics approach, integrated with a gene expression omnibus (GEO) data mining technique, was employed in this investigation. The results indicated that ZSD administration significantly alleviated LPS\u2010induced pathological changes in lung tissue. In the model group, the concentrations of interleukin\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    (IL\u20101\n                    <jats:italic>\u03b2<\/jats:italic>\n                    ), interleukin\u20106 (IL\u20106), and tumor necrosis factor\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    (TNF\u2010\n                    <jats:italic>\u03b1<\/jats:italic>\n                    ) were increased, whereas the levels of immunoglobulin A (IgA) and immunoglobulin M (IgM) were significantly decreased. These indicators were reversed considerably in the ZSD\u2010high dose (ZSD\u2010H) group. A total of 118 metabolites exhibiting significant alterations in lung tissue were identified through metabolomics analysis. Following ZSD treatment, 84 of these metabolites were negatively regulated, and the associated metabolic processes implicated multiple pathways, including sphingolipid and arachidonic acid (AA) metabolism. Differentially expressed genes (DEGs) were identified through a comprehensive analysis of GEO datasets. Integrative pathway analysis identified signaling pathways associated with ZSD treatment effects in acute pneumonia. Notably, the Janus kinase\/signal transducer and activator of transcription (JAK\u2010STAT) and Toll\u2010like receptor (TLR) signaling pathways emerged as critical contributors to these effects. This study indicates that ZSD can exert therapeutic effects on LPS\u2010induced acute pneumonia through a regulatory mechanism involving multiple components, targets, and pathways.\n                  <\/jats:p>","DOI":"10.1155\/mi\/6034066","type":"journal-article","created":{"date-parts":[[2026,5,11]],"date-time":"2026-05-11T10:43:03Z","timestamp":1778496183000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Lung Tissue Metabolome Investigation Reveals the In Vivo Effects of Zhuye Shigao Decoction in an LPS\u2010Induced Acute Pneumonia Model in Mice"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0005-3144","authenticated-orcid":false,"given":"Haipeng","family":"Tang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-8994-0021","authenticated-orcid":false,"given":"Zhiliang","family":"Sun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-6162-6087","authenticated-orcid":false,"given":"Weibo","family":"Qin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-0398-9938","authenticated-orcid":false,"given":"Guangzhi","family":"Cai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-2874-3216","authenticated-orcid":false,"given":"Wenyi","family":"Gao","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2582-7480","authenticated-orcid":false,"given":"Yang","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8573-1870","authenticated-orcid":false,"given":"Jiyu","family":"Gong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,5,11]]},"reference":[{"key":"e_1_2_11_1_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-021-00259-0"},{"key":"e_1_2_11_2_2","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.01672-2023"},{"key":"e_1_2_11_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)02724-0"},{"key":"e_1_2_11_4_2","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11122287"},{"key":"e_1_2_11_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/resp.13115"},{"key":"e_1_2_11_6_2","doi-asserted-by":"publisher","DOI":"10.1142\/S0192415X2050038X"},{"key":"e_1_2_11_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eujim.2020.101242"},{"key":"e_1_2_11_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11655-014-1754-1"},{"key":"e_1_2_11_9_2","doi-asserted-by":"publisher","DOI":"10.13729\/j.issn.1671-7813.Z20212245"},{"key":"e_1_2_11_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1875-5364(25)60903-4"},{"key":"e_1_2_11_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2024.08.017"},{"key":"e_1_2_11_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbad027"},{"key":"e_1_2_11_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbad346"},{"key":"e_1_2_11_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trac.2014.06.007"},{"key":"e_1_2_11_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.5614"},{"key":"e_1_2_11_16_2","doi-asserted-by":"publisher","DOI":"10.1039\/D2MO00216G"},{"key":"e_1_2_11_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.114280"},{"key":"e_1_2_11_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bty721"},{"key":"e_1_2_11_19_2","first-page":"614","article-title":"Characterization of the Chemical Composition of the Substance Benchmark of ZhuYe ShiGao Decoction Based on UHPLC-Q-Orbitrap\/MS","volume":"44","author":"Shen J.","year":"2023","journal-title":"Journal of Chinese Mass Spectrometry Society"},{"key":"e_1_2_11_20_2","doi-asserted-by":"publisher","DOI":"10.25259\/AJC_192_2024"},{"key":"e_1_2_11_21_2","doi-asserted-by":"publisher","DOI":"10.3389\/fphar.2023.1200058"},{"key":"e_1_2_11_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110177"},{"key":"e_1_2_11_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2016.01.016"},{"key":"e_1_2_11_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.14529"},{"key":"e_1_2_11_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13475"},{"key":"e_1_2_11_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s40265-017-0701-9"},{"key":"e_1_2_11_27_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12944-020-01373-w"},{"key":"e_1_2_11_28_2","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/8606878"},{"key":"e_1_2_11_29_2","doi-asserted-by":"publisher","DOI":"10.1161\/circ.142.suppl_3.15736"},{"key":"e_1_2_11_30_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-019-6367-9"},{"key":"e_1_2_11_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature13479"},{"key":"e_1_2_11_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/123.12.2174"},{"key":"e_1_2_11_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.11042"},{"key":"e_1_2_11_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2009.05.046"},{"key":"e_1_2_11_35_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20091240"},{"key":"e_1_2_11_36_2","doi-asserted-by":"publisher","DOI":"10.1124\/dmd.112.046466"},{"key":"e_1_2_11_37_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad9903"},{"key":"e_1_2_11_38_2","doi-asserted-by":"publisher","DOI":"10.1159\/000058445"},{"key":"e_1_2_11_39_2","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2023.1143583"},{"key":"e_1_2_11_40_2","first-page":"81","article-title":"Clinical Study of Using Zhuye Shigao Decoction Combined With Maimendong Decoction in the Treatment of Drugresistant Community-Acquired Pneumonia","volume":"35","author":"Li J.","year":"2017","journal-title":"Journal of Sichuan of Traditional Chinese Medicine"},{"key":"e_1_2_11_41_2","doi-asserted-by":"publisher","DOI":"10.4161\/cib.29053"},{"key":"e_1_2_11_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2020.165683"},{"key":"e_1_2_11_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.4613"},{"key":"e_1_2_11_44_2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/968083"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/mi\/6034066","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/mi\/6034066","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/mi\/6034066","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T10:43:16Z","timestamp":1778928196000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/mi\/6034066"}},"subtitle":[],"editor":[{"given":"Kimberly","family":"Jasmer","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":44,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/mi\/6034066"],"URL":"https:\/\/doi.org\/10.1155\/mi\/6034066","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-10-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-17","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"6034066"}}