{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T08:06:40Z","timestamp":1780646800829,"version":"3.54.1"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T00:00:00Z","timestamp":1780617600000},"content-version":"vor","delay-in-days":155,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100017594","name":"Medical Science and Technology Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2024ZL008"],"award-info":[{"award-number":["2024ZL008"]}],"id":[{"id":"10.13039\/501100017594","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017594","name":"Medical Science and Technology Project of Zhejiang Province","doi-asserted-by":"publisher","award":["2023ZR063"],"award-info":[{"award-number":["2023ZR063"]}],"id":[{"id":"10.13039\/501100017594","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Mediators of Inflammation"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Ferroptosis is involved in the pathogenesis of chronic kidney disease (CKD). Qingxin Lianzi Yin (QXLZY) has shown significant advantages in CKD treatment. This investigation intended to explore the impact of QXLZY on ferroptosis in CKD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The anti\u2010CKD effects of QXLZY were evaluated using the 5\/6 nephrectomy\u2010induced rat model and TGF\u2010\u03b21\u2010stimulated HK\u20102 cells. Oxidative stress and ferroptosis levels were assessed using commercial kits and western blot. The mechanism of QXLZY in CKD treatment was predicted through network pharmacology. TLR4 and HIF\u20101\u03b1 levels were detected by western blot. The role of ferroptosis in CKD treatment by QXLZY was explored using the ferroptosis inducer erastin. HK\u20102 cells overexpressing TLR4 were treated with the HIF\u20101\u03b1 inhibitor LW6 to investigate the underlying mechanism. QXLZY components identified through network pharmacology as potentially interacting with TLR4 were validated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>QXLZY effectively alleviated renal injury and inhibited fibrosis and inflammation in CKD models. Moreover, QXLZY markedly suppressed oxidative stress and ferroptosis. However, the anti\u2010CKD effects of QXLZY were partially counteracted by erastin. TLR4 and HIF\u20101\u03b1 were identified as potential targets through which QXLZY regulates ferroptosis in CKD. Their levels were notably increased in CKD models but were decreased after QXLZY treatment. Overexpression of TLR4 reversed the anti\u2010CKD effects of QXLZY, and this phenomenon was altered by LW6. Lauric acid (LA) and caprylic acid (CA) were bioactive components within QXLZY regulating the TLR4\/HIF\u20101\u03b1 pathway in CKD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>QXLZY inhibits ferroptosis by downregulating the TLR4\/HIF\u20101\u03b1 pathway, thereby alleviating renal fibrosis and improving CKD, with LA and CA potentially playing significant roles.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1155\/mi\/7440641","type":"journal-article","created":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T07:30:59Z","timestamp":1780644659000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Qingxin Lianzi Yin Improves Chronic Kidney Disease by Targeting Ferroptosis via the TLR4\/HIF\u20101\u03b1 Pathway"],"prefix":"10.1155","volume":"2026","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3925-6953","authenticated-orcid":false,"given":"Binqi","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Lin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6415-8585","authenticated-orcid":false,"given":"Danna","family":"Zheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-5948-7372","authenticated-orcid":false,"given":"Luxi","family":"Cao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,5]]},"reference":[{"key":"e_1_2_14_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(21)00519-5"},{"key":"e_1_2_14_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj-2022-074216"},{"key":"e_1_2_14_3_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-019-1856-9"},{"key":"e_1_2_14_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41422-020-00441-1"},{"key":"e_1_2_14_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2024.111256"},{"key":"e_1_2_14_6_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12964-023-01422-8"},{"key":"e_1_2_14_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-023-00735-1"},{"key":"e_1_2_14_8_2","doi-asserted-by":"publisher","DOI":"10.19664\/j.cnki.1002-2392.220235"},{"key":"e_1_2_14_9_2","unstructured":"DingH. Clinical Study on Qingxin Lianzi Decoction in Treating IgA Nephropathy With Deficiency of Qi and Yin 2023."},{"key":"e_1_2_14_10_2","unstructured":"GaoW. Study on the Chemical Material Basis of Chinese Classical Formula Qingxin Lianzi Yin Decoction and Evaluation of Its Efficacy in Diabetic Nephropathy Model 2021."},{"key":"e_1_2_14_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12979-023-00383-3"},{"key":"e_1_2_14_12_2","doi-asserted-by":"publisher","DOI":"10.14814\/phy2.12558"},{"key":"e_1_2_14_13_2","doi-asserted-by":"publisher","DOI":"10.3109\/0886022X.2016.1143757"},{"key":"e_1_2_14_14_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.12.7516"},{"key":"e_1_2_14_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2025.156738"},{"key":"e_1_2_14_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1758-2946-6-13"},{"key":"e_1_2_14_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/cpbi.5"},{"key":"e_1_2_14_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/database\/bav028"},{"key":"e_1_2_14_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku1205"},{"key":"e_1_2_14_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkac935"},{"key":"e_1_2_14_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.xinn.2021.100141"},{"key":"e_1_2_14_22_2","doi-asserted-by":"publisher","DOI":"10.1080\/0886022X.2024.2375033"},{"key":"e_1_2_14_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2022.121331"},{"key":"e_1_2_14_24_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.14745"},{"key":"e_1_2_14_25_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00398.2021"},{"key":"e_1_2_14_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41581-023-00694-0"},{"key":"e_1_2_14_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-023-01156-w"},{"key":"e_1_2_14_28_2","first-page":"778","article-title":"Clinical Effects of Modified Qingxin Lianzi Decoction on Diabetic Kidney Disease at Stage III to IV with Deficiency of Both Qi and Yin","volume":"25","author":"Zhang L.","year":"2024","journal-title":"Chinese Journal of Integrated Traditional and Western Nephrology"},{"key":"e_1_2_14_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2023.155222"},{"key":"e_1_2_14_30_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines10020420"},{"key":"e_1_2_14_31_2","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines11092358"},{"key":"e_1_2_14_32_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms18050950"},{"key":"e_1_2_14_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-023-43226-x"},{"key":"e_1_2_14_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12020-017-1402-6"},{"key":"e_1_2_14_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41401-023-01070-1"},{"key":"e_1_2_14_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2024.118679"},{"key":"e_1_2_14_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2024.155632"},{"key":"e_1_2_14_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2021.113917"}],"container-title":["Mediators of Inflammation"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/mi\/7440641","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1155\/mi\/7440641","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/mi\/7440641","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T07:31:04Z","timestamp":1780644664000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/mi\/7440641"}},"subtitle":[],"editor":[{"given":"Diogo","family":"Peruchetti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,1]]},"references-count":38,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,1]]}},"alternative-id":["10.1155\/mi\/7440641"],"URL":"https:\/\/doi.org\/10.1155\/mi\/7440641","archive":["Portico"],"relation":{},"ISSN":["0962-9351","1466-1861"],"issn-type":[{"value":"0962-9351","type":"print"},{"value":"1466-1861","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,1]]},"assertion":[{"value":"2025-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-05-16","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"7440641"}}